• Western blot analysis of extracts of various cell lines, using IFNG antibody (STJ114324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry of paraffin-embedded human lung using IFNG antibody (STJ114324) at dilution of 1:100 (40x lens).

Anti-IFNG antibody (24-161) (STJ114324)

SKU:
STJ114324

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IFNG (24-161) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFNG
Gene ID: 3458
Uniprot ID: IFNG_HUMAN
Immunogen Region: 24-161
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-161 of IFN gamma (NP_997468.1).
Immunogen Sequence: VFGISYDSPDYTDESCTFKI SLRNFRSILSWELKNHSIVP THYTLLYTIMSKPEDLKVVK NCANTTRSFCDLTDEWRSTH EAYVTVLEGFSGNTTLFSCS HNFWLAIDMSFEPPEFEIVG FTNHINVMVKFPSIVEEE
Tissue Specificity Released primarily from activated T lymphocytes.
Post Translational Modifications Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
Function Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
Protein Name Interferon Gamma
Ifn-Gamma
Immune Interferon
Database Links Reactome: R-HSA-877300
Reactome: R-HSA-877312
Reactome: R-HSA-8877330
Reactome: R-HSA-8950505
Reactome: R-HSA-9732724
Cellular Localisation Secreted
Alternative Antibody Names Anti-Interferon Gamma antibody
Anti-Ifn-Gamma antibody
Anti-Immune Interferon antibody
Anti-IFNG antibody

Information sourced from Uniprot.org

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