• Western blot analysis of extracts of various cell lines, using IL12RB2 antibody (STJ112386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-IL12RB2 antibody (420-620) (STJ112386)

SKU:
STJ112386

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL12RB2 (420-620) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL12RB2
Gene ID: 3595
Uniprot ID: I12R2_HUMAN
Immunogen Region: 420-620
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 420-620 of human IL12RB2 (NP_001550.1).
Immunogen Sequence: GLLAPRQVSANSEGMDNILV TWQPPRKDPSAVQEYVVEWR ELHPGGDTQVPLNWLRSRPY NVSALISENIKSYICYEIRV YALSGDQGGCSSILGNSKHK APLSGPHINAITEEKGSILI SWNSIPVQEQMGCLLHYRIY WKERDSNSQPQLCEIPYRVS QNSHPINSLQPRVTYVLWMT ALTAAGESSHGNEREFCLQG K
Tissue Specificity Isoform 2 is expressed at similar levels in both naive and activated T-cells.
Post Translational Modifications On IL12 binding, phosphorylated on C-terminal tyrosine residues by JAK2. Phosphorylation on Tyr-800 is required for STAT4 binding and activation, and for SOCS3 binding.
Function Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
Protein Name Interleukin-12 Receptor Subunit Beta-2
Il-12 Receptor Subunit Beta-2
Il-12r Subunit Beta-2
Il-12r-Beta-2
Il-12rb2
Database Links Reactome: R-HSA-8984722
Reactome: R-HSA-9020591
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Interleukin-12 Receptor Subunit Beta-2 antibody
Anti-Il-12 Receptor Subunit Beta-2 antibody
Anti-Il-12r Subunit Beta-2 antibody
Anti-Il-12r-Beta-2 antibody
Anti-Il-12rb2 antibody
Anti-IL12RB2 antibody

Information sourced from Uniprot.org

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