• Western blot analysis of various lysates, using IL13RA2 antibody (STJ28140) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of Rat uterus, using IL13RA2 antibody (STJ28140) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using IL13RA2 rabbit polyclonal antibody (STJ28140) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human liver cancer using IL13RA2 rabbit polyclonal antibody (STJ28140) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using IL13RA2 rabbit polyclonal antibody (STJ28140) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat spleen using IL13RA2 rabbit polyclonal antibody (STJ28140) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-IL13RA2 antibody (27-343) (STJ28140)

SKU:
STJ28140

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL13RA2 (27-343) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL13RA2
Gene ID: 3598
Uniprot ID: I13R2_HUMAN
Immunogen Region: 27-343
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-343 of human IL13RA2 (NP_000631.1).
Immunogen Sequence: DTEIKVNPPQDFEIVDPGYL GYLYLQWQPPLSLDHFKECT VEYELKYRNIGSETWKTIIT KNLHYKDGFDLNKGIEAKIH TLLPWQCTNGSEVQSSWAET TYWISPQGIPETKVQDMDCV YYNWQYLLCSWKPGIGVLLD TNYNLFYWYEGLDHALQCVD YIKADGQNIGCRFPYLEASD YKDFYICVNGSSENKPIRSS YFTFQLQNIVKPLPPVYLTF TRESSCEIKLKWSIPLGPI
Function Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
Protein Name Interleukin-13 Receptor Subunit Alpha-2
Il-13 Receptor Subunit Alpha-2
Il-13r Subunit Alpha-2
Il-13r-Alpha-2
Il-13ra2
Interleukin-13-Binding Protein
Cd Antigen Cd213a2
Database Links Reactome: R-HSA-6785807
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Interleukin-13 Receptor Subunit Alpha-2 antibody
Anti-Il-13 Receptor Subunit Alpha-2 antibody
Anti-Il-13r Subunit Alpha-2 antibody
Anti-Il-13r-Alpha-2 antibody
Anti-Il-13ra2 antibody
Anti-Interleukin-13-Binding Protein antibody
Anti-Cd Antigen Cd213a2 antibody
Anti-IL13RA2 antibody
Anti-IL13R antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance