• Western blot analysis of extracts of NCI-H460 cells, using IL18R1/IL-18 R alpha antibody (STJ24172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using IL18R1/IL-18 R alpha rabbit polyclonal antibody (STJ24172) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using IL18R1/IL-18 R alpha rabbit polyclonal antibody (STJ24172) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-IL18R1 antibody (19-320) (STJ24172)

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STJ24172

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL18R1 (19-320) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL18R1
Gene ID: 8809
Uniprot ID: IL18R_HUMAN
Immunogen Region: 19-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-320 of human IL18R1/IL-18 R alpha (NP_003846.1).
Immunogen Sequence: AESCTSRPHITVVEGEPFYL KHCSCSLAHEIETTTKSWYK SSGSQEHVELNPRSSSRIAL HDCVLEFWPVELNDTGSYFF QMKNYTQKWKLNVIRRNKHS CFTERQVTSKIVEVKKFFQI TCENSYYQTLVNSTSLYKNC KKLLLENNKNPTIKKNAEFE DQGYYSCVHFLHHNGKLFNI TKTFNITIVEDRSNIVPVLL GPKLNHVAVELGKNVRLNCS ALLNEEDVIYWMFGEENGS
Tissue Specificity Highly expressed in leukocytes, spleen, lung. Also expressed, but at lower levels, in liver, small intestine, colon, prostate, thymus, placenta, and heart. Specifically coexpressed with IL18R1 in Th1 cells.
Post Translational Modifications N-glycosylated. N-linked glycosyl chains contribute to ligand recognition and intra-receptor interactions required for formation of an active ternary receptor complex.
Function Within the IL18 receptor complex, responsible for the binding of the pro-inflammatory cytokine IL18, but not IL1A nor IL1B. Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells. Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3.
Protein Name Interleukin-18 Receptor 1
Il-18r-1
Il-18r1
Cd218 Antigen-Like Family Member A
Cdw218a
Il1 Receptor-Related Protein
Il-1rrp
Il1r-Rp
Interleukin-18 Receptor Alpha
Il-18r-Alpha
Il-18ralpha
Cd Antigen Cd218a
Database Links Reactome: R-HSA-9008059
Reactome: R-HSA-9012546
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Interleukin-18 Receptor 1 antibody
Anti-Il-18r-1 antibody
Anti-Il-18r1 antibody
Anti-Cd218 Antigen-Like Family Member A antibody
Anti-Cdw218a antibody
Anti-Il1 Receptor-Related Protein antibody
Anti-Il-1rrp antibody
Anti-Il1r-Rp antibody
Anti-Interleukin-18 Receptor Alpha antibody
Anti-Il-18r-Alpha antibody
Anti-Il-18ralpha antibody
Anti-Cd Antigen Cd218a antibody
Anti-IL18R1 antibody
Anti-IL1RRP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance