• Western blot analysis of extracts of various cell lines, using IL1R1 antibody (STJ28294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL1R1 antibody (380-569) (STJ28294)

SKU:
STJ28294

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL1R1 (380-569) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL1R1
Gene ID: 3554
Uniprot ID: IL1R1_HUMAN
Immunogen Region: 380-569
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 380-569 of human IL1R1 (NP_000868.1).
Immunogen Sequence: SDGKTYDAYILYPKTVGEGS TSDCDIFVFKVLPEVLEKQC GYKLFIYGRDDYVGEDIVEV INENVKKSRRLIIILVRETS GFSWLGGSSEEQIAMYNALV QDGIKVVLLELEKIQDYEKM PESIKFIKQKHGAIRWSGDF TQGPQSAKTRFWKNVRYHMP VQRRSPSSKHQLLSPATKEK LQREAHVPLG
Tissue Specificity Expressed in T-helper cell subsets. Preferentially expressed in T-helper 1 (Th1) cells.
Post Translational Modifications A soluble form (sIL1R1) is probably produced by proteolytic cleavage at the cell surface (shedding). Rapidly phosphorylated on Tyr-496 in response to IL-1, which creates a SH2 binding site for the PI 3-kinase regulatory subunit PIK3R1.
Function Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.
Protein Name Interleukin-1 Receptor Type 1
Il-1r-1
Il-1rt-1
Il-1rt1
Cd121 Antigen-Like Family Member A
Interleukin-1 Receptor Alpha
Il-1r-Alpha
Interleukin-1 Receptor Type I
P80
Cd Antigen Cd121a Cleaved Into - Interleukin-1 Receptor Type 1 - Membrane Form
Mil-1r1
Mil-1ri - Interleukin-1 Receptor Type 1 - Soluble Form
Sil-1r1
Sil-1ri
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-9020702
Reactome: R-HSA-9679191
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cell Membrane
Secreted
Alternative Antibody Names Anti-Interleukin-1 Receptor Type 1 antibody
Anti-Il-1r-1 antibody
Anti-Il-1rt-1 antibody
Anti-Il-1rt1 antibody
Anti-Cd121 Antigen-Like Family Member A antibody
Anti-Interleukin-1 Receptor Alpha antibody
Anti-Il-1r-Alpha antibody
Anti-Interleukin-1 Receptor Type I antibody
Anti-P80 antibody
Anti-Cd Antigen Cd121a Cleaved Into - Interleukin-1 Receptor Type 1 - Membrane Form antibody
Anti-Mil-1r1 antibody
Anti-Mil-1ri - Interleukin-1 Receptor Type 1 - Soluble Form antibody
Anti-Sil-1r1 antibody
Anti-Sil-1ri antibody
Anti-IL1R1 antibody
Anti-IL1R antibody
Anti-IL1RA antibody
Anti-IL1RT1 antibody

Information sourced from Uniprot.org

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