• Western blot analysis of extracts of various cell lines, using IL1R2 antibody (STJ116007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IL1R2 antibody (20-320) (STJ116007)

SKU:
STJ116007

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IL1R2 (20-320) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IL1R2
Gene ID: 7850
Uniprot ID: IL1R2_HUMAN
Immunogen Region: 20-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human IL1R2 (NP_775465.1).
Immunogen Sequence: AHTGAARSCRFRGRHYKREF RLEGEPVALRCPQVPYWLWA SVSPRINLTWHKNDSARTVP GEEETRMWAQDGALWLLPAL QEDSGTYVCTTRNASYCDKM SIELRVFENTDAFLPFISYP QILTLSTSGVLVCPDLSEFT RDKTDVKIQWYKDSLLLDKD NEKFLSVRGTTHLLVHDVAL EDAGYYRCVLTFAHEGQQYN ITRSIELRIKKKKEETIPVI ISPLKTISASLGSRLTIPC
Post Translational Modifications A soluble form (sIL1R2) can also be produced by proteolytic cleavage at the cell surface (shedding) involving a metalloproteinase.hovever, several sIL1R2 forms ranging from 45 and 60 kDa are reported.
Function Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity.this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Protein Name Interleukin-1 Receptor Type 2
Il-1r-2
Il-1rt-2
Il-1rt2
Cd121 Antigen-Like Family Member B
Cdw121b
Il-1 Type Ii Receptor
Interleukin-1 Receptor Beta
Il-1r-Beta
Interleukin-1 Receptor Type Ii
Cd Antigen Cd121b Cleaved Into - Interleukin-1 Receptor Type 2 - Membrane Form
Mil-1r2
Mil-1rii - Interleukin-1 Receptor Type 2 - Soluble Form
Sil-1r2
Sil-1rii
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-9020702
Cellular Localisation Isoform Short: Secreted
Isoform Long: Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Interleukin-1 Receptor Type 2 antibody
Anti-Il-1r-2 antibody
Anti-Il-1rt-2 antibody
Anti-Il-1rt2 antibody
Anti-Cd121 Antigen-Like Family Member B antibody
Anti-Cdw121b antibody
Anti-Il-1 Type Ii Receptor antibody
Anti-Interleukin-1 Receptor Beta antibody
Anti-Il-1r-Beta antibody
Anti-Interleukin-1 Receptor Type Ii antibody
Anti-Cd Antigen Cd121b Cleaved Into - Interleukin-1 Receptor Type 2 - Membrane Form antibody
Anti-Mil-1r2 antibody
Anti-Mil-1rii - Interleukin-1 Receptor Type 2 - Soluble Form antibody
Anti-Sil-1r2 antibody
Anti-Sil-1rii antibody
Anti-IL1R2 antibody
Anti-IL1RB antibody

Information sourced from Uniprot.org

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