| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-IL36A (1-158) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
IL36A |
| Gene ID: |
27179 |
| Uniprot ID: |
IL36A_HUMAN |
| Immunogen Region: |
1-158 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human IL36A (NP_055255.1). |
| Immunogen Sequence: |
MEKALKIDTPQQGSIQDINH RVWVLQDQTLIAVPRKDRMS PVTIALISCRHVETLEKDRG NPIYLGLNGLNLCLMCAKVG DQPTLQLKEKDIMDLYNQPE PVKSFLFYHSQSGRNSTFES VAFPGWFIAVSSEGGCPLIL TQELGKANTTDFGLTMLF |
| Tissue Specificity | Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin. |
| Post Translational Modifications | N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). |
| Function | Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response.similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of pro-inflammatory cytokines such as TNF-alpha, IL-8 and IL-6. In cultured monocytes up-regulates expression of IL-1A, IL-1B and IL-6. In myeloid dendritic cells involved in cell maturation by up-regulating surface expression of CD83, CD86 and HLA-DR. In monocyte-derived dendritic cells facilitates dendritic cell maturation and drives T-cell proliferation. May play a role in pro-inflammatory effects in the lung. |
| Protein Name | Interleukin-36 AlphaFil1 EpsilonInterleukin-1 EpsilonIl-1 EpsilonInterleukin-1 Family Member 6Il-1f6 |
| Database Links | Reactome: R-HSA-9014826 |
| Cellular Localisation | CytoplasmSecretedThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Antibody Names | Anti-Interleukin-36 Alpha antibodyAnti-Fil1 Epsilon antibodyAnti-Interleukin-1 Epsilon antibodyAnti-Il-1 Epsilon antibodyAnti-Interleukin-1 Family Member 6 antibodyAnti-Il-1f6 antibodyAnti-IL36A antibodyAnti-FIL1E antibodyAnti-IL1E antibodyAnti-IL1F6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
