| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-JAM3 (1-100) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
JAM3 |
| Gene ID: |
83700 |
| Uniprot ID: |
JAM3_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human JAM3 (NP_116190.3). |
| Immunogen Sequence: |
MALRRPPRLRLCARLPDFFL LLLFRGCLIGAVNLKSSNRT PVVQEFESVELSCIITDSQT SDPRIEWKKIQDEQTTYVFF DNKIQGDLAGRAEILGKTSL |
| Tissue Specificity | Detected on round and elongated spermatids (at protein level). Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level). |
| Post Translational Modifications | Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17.the release of soluble JAM3 is increased by pro-inflammatory factors. S-palmitoylated by ZDHHC7. S-palmitoylation promotes expression at tight junctions. |
| Function | Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM2 to regulate different cellular processes. Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3. Plays a central role in leukocytes extravasation by facilitating transmigration through the endothelium. Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation. Also functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). Plays a role in angiogenesis. Plays a role in the regulation of cell migration (Probable). During myogenesis, it is involved in myocyte fusion. Soluble form of JAM-C: Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis. |
| Protein Name | Junctional Adhesion Molecule CJam-CJam-2Junctional Adhesion Molecule 3Jam-3 Cleaved Into - Soluble Form Of Jam-CSjam-C |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-216083 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionDesmosomeTight JunctionDetected In The Acrosome Region In Developing SpermatidsIn Epithelial CellsIt Is Expressed At Desmosomes But Not At Tight JunctionsLocalizes At The Cell Surface Of Endothelial CellsTreatment Of Endothelial Cells With Vascular Endothelial Growth Factor Stimulates Recruitment Of Jam3 To Cell-Cell ContactsSoluble Form Of Jam-C: Secreted |
| Alternative Antibody Names | Anti-Junctional Adhesion Molecule C antibodyAnti-Jam-C antibodyAnti-Jam-2 antibodyAnti-Junctional Adhesion Molecule 3 antibodyAnti-Jam-3 Cleaved Into - Soluble Form Of Jam-C antibodyAnti-Sjam-C antibodyAnti-JAM3 antibodyAnti-UNQ859 antibodyAnti-PRO1868 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
