• Western blot analysis of extracts of various cell lines, using KCNK1 antibody (STJ114701) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-KCNK1 antibody (267-336) (STJ114701)

SKU:
STJ114701

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KCNK1 (267-336) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KCNK1
Gene ID: 3775
Uniprot ID: KCNK1_HUMAN
Immunogen Region: 267-336
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 267-336 of human KCNK1 (NP_002236.1).
Immunogen Sequence: FCELHELKKFRKMFYVKKDK DEDQVHIIEHDQLSFSSITD QAAGMKEDQKQNEPFVATQS SACVDGPANH
Tissue Specificity Detected in bronchial epithelial cells. Detected in heart left atrium and left ventricle. Detected in cardiac myocytes (at protein level). Widely expressed with high levels in heart, brain and kidney, and lower levels in colon, ovary, placenta, lung and liver. Highly expressed in cerebellum, and detected at lower levels in amygdala, caudate nucleus, brain cortex, hippocampus, putamen, substantia nigra, thalamus, dorsal root ganglion, spinal cord, pituitary, heart, kidney, lung, placenta, pancreas, stomach, small intestine, uterus and prostate. Detected in right and left heart ventricle and atrium, and in heart Purkinje fibers. Detected in bronchial epithelial cells.
Post Translational Modifications Sumoylation is controversial. Sumoylated by UBE2I. Not sumoylated when expressed in xenopus oocytes or mammalian cells. Sumoylation inactivates the channel, but does not interfere with expression at the cell membrane. Sumoylation of a single subunit is sufficient to silence the dimeric channel. Sumoylation of KCNK1 is sufficient to silence heterodimeric channels formed by KCNK1 and KCNK3 or KCNK9. Desumoylated by SENP1.this activates the channel. Desumoylated by SENP1.this strongly increases halothane-mediated activation of heterodimeric channels formed with KCNK9. SENP1 treatment has no effect.
Function Ion channel that contributes to passive transmembrane potassium transport and to the regulation of the resting membrane potential in brain astrocytes, but also in kidney and in other tissues. Forms dimeric channels through which potassium ions pass in accordance with their electrochemical gradient. The channel is selective for K(+) ions at physiological potassium concentrations and at neutral pH, but becomes permeable to Na(+) at subphysiological K(+) levels and upon acidification of the extracellular medium. The homodimer has very low potassium channel activity, when expressed in heterologous systems, and can function as weakly inward rectifying potassium channel. Channel activity is modulated by activation of serotonin receptors. Heterodimeric channels containing KCNK1 and KCNK2 have much higher activity, and may represent the predominant form in astrocytes. Heterodimeric channels containing KCNK1 and KCNK3 or KCNK9 have much higher activity. Heterodimeric channels formed by KCNK1 and KCNK9 may contribute to halothane-sensitive currents. Mediates outward rectifying potassium currents in dentate gyrus granule cells and contributes to the regulation of their resting membrane potential. Contributes to the regulation of action potential firing in dentate gyrus granule cells and down-regulates their intrinsic excitability. In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1. Required for normal ion and water transport in the kidney. Contributes to the regulation of the resting membrane potential of pancreatic beta cells. The low channel activity of homodimeric KCNK1 may be due to sumoylation. The low channel activity may be due to rapid internalization from the cell membrane and retention in recycling endosomes.
Protein Name Potassium Channel Subfamily K Member 1
Inward Rectifying Potassium Channel Protein Twik-1
Potassium Channel K2p1
Potassium Channel Kcno1
Database Links Reactome: R-HSA-1299308
Reactome: R-HSA-5576886
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Recycling Endosome
Synaptic Cell Membrane
Cytoplasmic Vesicle
Perikaryon
Cell Projection
Dendrite
Apical Cell Membrane
The Heterodimer With Kcnk2 Is Detected At The Astrocyte Cell Membrane
Not Detected At The Astrocyte Cell Membrane When Kcnk2 Is Absent
Detected On Neuronal Cell Bodies
And To A Lesser Degree On Neuronal Cell Projections
Detected On Hippocampus Dentate Gyrus Granule Cell Bodies And To A Lesser Degree On Proximal Dendrites
Detected At The Apical Cell Membrane In Stria Vascularis In The Cochlea
Detected At The Apical Cell Membrane Of Vestibular Dark Cells Situated Between The Crista And The Utricle In The Inner Ear
Detected At The Apical Cell Membrane In Kidney Proximal Tubule Segment S1 And In Subapical Compartments In Segments S1
S2 And S3
Predominantly In Cytoplasmic Structures In Kidney Distal Convoluted Tubules And Collecting Ducts
Detected At The Apical Cell Membrane Of Bronchial Epithelial Cells
Alternative Antibody Names Anti-Potassium Channel Subfamily K Member 1 antibody
Anti-Inward Rectifying Potassium Channel Protein Twik-1 antibody
Anti-Potassium Channel K2p1 antibody
Anti-Potassium Channel Kcno1 antibody
Anti-KCNK1 antibody
Anti-HOHO1 antibody
Anti-KCNO1 antibody
Anti-TWIK1 antibody

Information sourced from Uniprot.org

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