• Western blot analysis of extracts of various cell lines, using KIF20B antibody (STJ117555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KIF20B antibody (1200-1500) (STJ117555)

SKU:
STJ117555

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIF20B (1200-1500) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF20B
Gene ID: 9585
Uniprot ID: KI20B_HUMAN
Immunogen Region: 1200-1500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1500 of human KIF20B (NP_057279.2).
Immunogen Sequence: KHLLQLKEEEEETNRQETEK LKEELSASSARTQNLKADLQ RKEEDYADLKEKLTDAKKQI KQVQKEVSVMRDEDKLLRIK INELEKKKNQCSQELDMKQR TIQQLKEQLNNQKVEEAIQQ YERACKDLNVKEKIIEDMRM TLEEQEQTQVEQDQVLEAKL EEVERLATELEKWKEKCNDL ETKNNQRSNKEHENNTDVLG KLTNLQDELQESEQKYNADR KKWLEEKMMLITQAKEAEN
Tissue Specificity Brain, ovary, kidney and testis (at protein level). Overexpressed in bladder cancer cells (at protein level). Expressed in testis. Overexpressed in bladder cancer cells.
Post Translational Modifications Phosphorylated during mitosis by CDK1.
Function Plus-end-directed motor enzyme that is required for completion of cytokinesis. Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.
Protein Name Kinesin-Like Protein Kif20b
Cancer/Testis Antigen 90
Ct90
Kinesin Family Member 20b
Kinesin-Related Motor Interacting With Pin1
M-Phase Phosphoprotein 1
Mpp1
Database Links Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleolus
Nucleoplasm
Spindle
Spindle Pole
Midbody
Cell Projection
Axon
Growth Cone
Localizes Mainly In The Nucleus During Interphase Although It Is Also Detected In The Cytoplasm Without Clear Association With Microtubules
Localized To The Central Spindle During Cytokinetic Furrowing And With The Midbody During Abscission
A 2-3 Fold Expression Increase Is Seen As Cells Progress From G1 To G2/M Phase
During Prophase And Metaphase It Is Found Throughout The Cytoplasm And At Anaphase Accumulates At The Midplan Of The Cell And Forms A Distinct Band Extending Across The Spindle Midzone
At Anaphase It Is Concentrated In The Midbody
Colocalized Partially Along Microtubules In Primary Neurons
Colocalized With Shtn1 Along Microtubules To The Tip Of The Growing Cone In Primary Hippocampal Neurons
Localized In Midbodies Between Dividing Radial Progenitors In The Ventricular Zone
Colocalized With Prc1 In The Nucleus Of Bladder Carcinoma Cells At The Interphase
Colocalized With Prc1 In Bladder Carcinoma Cells At Prophase
Metaphase
Early Anaphase
At The Midzone In Late Anaphase And At The Contractile Ring In Telophase
Alternative Antibody Names Anti-Kinesin-Like Protein Kif20b antibody
Anti-Cancer/Testis Antigen 90 antibody
Anti-Ct90 antibody
Anti-Kinesin Family Member 20b antibody
Anti-Kinesin-Related Motor Interacting With Pin1 antibody
Anti-M-Phase Phosphoprotein 1 antibody
Anti-Mpp1 antibody
Anti-KIF20B antibody
Anti-KRMP1 antibody
Anti-MPHOSPH1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance