• Western blot analysis of extracts of MCF7 cells, using KRT10 antibody (STJ110217) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-KRT10 antibody (350-460) (STJ110217)

SKU:
STJ110217

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KRT10 (350-460) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT10
Gene ID: 3858
Uniprot ID: K1C10_HUMAN
Immunogen Region: 350-460
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-460 of human KRT10 (NP_000412.3).
Immunogen Sequence: EIDNNIEQISSYKSEITELR RNVQALEIELQSQLALKQSL EASLAETEGRYCVQLSQIQA QISALEEQLQQIRAETECQN TEYQQLLDIKIRLENEIQTY RSLLEGEGSSG
Tissue Specificity Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines. Localized on the surface of desquamated nasal epithelial cells (at protein level).
Function Plays a role in the establishment of the epidermal barrier on plantar skin. Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.
Protein Name Keratin - Type I Cytoskeletal 10
Cytokeratin-10
Ck-10
Keratin-10
K10
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Secreted
Extracellular Space
Cell Surface
Cytoplasm
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 10 antibody
Anti-Cytokeratin-10 antibody
Anti-Ck-10 antibody
Anti-Keratin-10 antibody
Anti-K10 antibody
Anti-KRT10 antibody
Anti-KPP antibody

Information sourced from Uniprot.org

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