• Western blot analysis of extracts of various cell lines, using Cytokeratin 4 (Cytokeratin 4 (KRT4) ) antibody (STJ114049) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-KRT4 antibody (446-520) (STJ114049)

SKU:
STJ114049

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KRT4 (446-520) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT4
Gene ID: 3851
Uniprot ID: K2C4_HUMAN
Immunogen Region: 446-520
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 446-520 of human Cytokeratin 4 (Cytokeratin 4 (KRT4) ) (NP_002263.3).
Immunogen Sequence: EEYRMSGECQSAVSISVVSG STSTGGISGGLGSGSGFGLS SGFGSGSGSGFGFGGSVSGS SSSKIISTTTLNKRR
Tissue Specificity Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
Protein Name Keratin - Type Ii Cytoskeletal 4
Cytokeratin-4
Ck-4
Keratin-4
K4
Type-Ii Keratin Kb4
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 4 antibody
Anti-Cytokeratin-4 antibody
Anti-Ck-4 antibody
Anti-Keratin-4 antibody
Anti-K4 antibody
Anti-Type-Ii Keratin Kb4 antibody
Anti-KRT4 antibody
Anti-CYK4 antibody

Information sourced from Uniprot.org

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