Anti-Ku-70 (Acetyl K539) antibody

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Product name Anti-Ku-70 (Acetyl K539) antibody
Short Description Rabbit polyclonal against Ku-70 (Acetyl K539)
Description Rabbit polyclonal to Acetyl-Ku-70 (K539).
Applications ELISA, IHC, WB
Dilution range WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
Specificity Acetyl-Ku-70 (K539) Polyclonal Antibody detects endogenous levels of Ku-70 protein only when acetylated at K539.
Protein Name X-ray repair cross-complementing protein 6
5'-deoxyribose-5-phosphate lyase Ku70
5'-dRP lyase Ku70
70 kDa subunit of Ku antigen
ATP-dependent DNA helicase 2 subunit 1
ATP-dependent DNA helicase II 70 kDa subunit
CTC box
Immunogen Synthesized peptide derived from the human Ku-70 around the acetylation site of K539.
Immunogen Region acetylation site of K539.
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IHC
Host Rabbit
Clonality Polyclonal
Reactivity Human
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 2547
Gene Symbol XRCC6
Molecular Weight 70 kDa
Database Links HGNC:4055
OMIM:152690
Alternative Names X-ray repair cross-complementing protein 6
5'-deoxyribose-5-phosphate lyase Ku70
5'-dRP lyase Ku70
70 kDa subunit of Ku antigen
ATP-dependent DNA helicase 2 subunit 1
ATP-dependent DNA helicase II 70 kDa subunit
CTC box
Function Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Post-translational Modifications Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
Cellular Localization Nucleus Chromosome
Swiss-Prot Key P12956_HUMAN
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Olivier Boucherat, Sophie Chabot, Roxane Paulin, Isabelle Trinh, Alice Bourgeois, François Potus, Marie-Claude Lampron, Caroline Lambert, Sandra Breuils-Bonnet, Valérie Nadeau, Renée Paradis, Elena A. Goncharova, Steeve Provencher & Sébastien Bonnet. HDAC6: A Novel Histone Deacetylase Implicated in Pulmonary Arterial Hypertension. Scientific Reportsvolume 7, Article number: 4546 (2017) doi:10.1038/s41598-017-04874-4 

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