• Immunofluorescence analysis of NIH/3T3 cells using LDL Receptor (LDLR) Rabbit polyclonal antibody (STJ117194) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of LDL Receptor (LDLR) in paraffin-embedded rat kidney using LDL Receptor (LDLR) Rabbit polyclonal antibody (STJ117194) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat brain, using LDL Receptor (LDLR) Rabbit polyclonal antibody (STJ117194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using LDL Receptor (LDLR) Rabbit polyclonal antibody (STJ117194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-LDLR antibody (761-860) (STJ117194)

SKU:
STJ117194

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LDLR (761-860) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDLR
Gene ID: 3949
Uniprot ID: LDLR_HUMAN
Immunogen Region: 761-860
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1).
Immunogen Sequence: TVEIVTMSHQALGDVAGRGN EKKPSSVRALSIVLPIVLLV FLCLGVFLLWKNWRLKNINS INFDNPVYQKTTEDEVHICH NQDGYSYPSRQMVSLEDDVA
Post Translational Modifications N- and O-glycosylated. Ubiquitinated by MYLIP leading to degradation.
Function Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
Protein Name Low-Density Lipoprotein Receptor
Ldl Receptor
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8964026
Reactome: R-HSA-8964038
Reactome: R-HSA-975634
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane
Clathrin-Coated Pit
Golgi Apparatus
Early Endosome
Late Endosome
Lysosome
Rapidly Endocytosed Upon Ligand Binding
Alternative Antibody Names Anti-Low-Density Lipoprotein Receptor antibody
Anti-Ldl Receptor antibody
Anti-LDLR antibody

Information sourced from Uniprot.org

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