• Western blot analysis of extracts of mouse brain, using LNPEP antibody (STJ113875) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Rat brain, using LNPEP antibody (STJ113875) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded rat liver using LNPEP rabbit polyclonal antibody (STJ113875) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat brain using LNPEP rabbit polyclonal antibody (STJ113875) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human placenta using LNPEP rabbit polyclonal antibody (STJ113875) at dilution of 1:100 (40x lens).

Anti-LNPEP antibody (1-110) (STJ113875)

SKU:
STJ113875

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LNPEP (1-110) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LNPEP
Gene ID: 4012
Uniprot ID: LCAP_HUMAN
Immunogen Region: 1-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human LNPEP (NP_005566.2).
Immunogen Sequence: MEPFTNDRLQLPRNMIENSM FEEEPDVVDLAKEPCLHPLE PDEVEYEPRGSRLLVRGLGE HEMEEDEEDYESSAKLLGMS FMNRSSGLRNSATGYRQSPD GACSVPSART
Tissue Specificity Highly expressed in placenta, heart, kidney and small intestine. Detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon.
Post Translational Modifications The pregnancy serum form is derived from the membrane-bound form by proteolytic processing. N-glycosylated.
Function Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.
Protein Name Leucyl-Cystinyl Aminopeptidase
Cystinyl Aminopeptidase
Insulin-Regulated Membrane Aminopeptidase
Insulin-Responsive Aminopeptidase
Irap
Oxytocinase
Otase
Placental Leucine Aminopeptidase
P-Lap Cleaved Into - Leucyl-Cystinyl Aminopeptidase - Pregnancy Serum Form
Database Links Reactome: R-HSA-1236977
Reactome: R-HSA-1445148
Reactome: R-HSA-983168
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
In Brain Only The Membrane-Bound Form Is Found
The Protein Resides In Intracellular Vesicles Together With Glut4 And Can Then Translocate To The Cell Surface In Response To Insulin And/Or Oxytocin
Localization May Be Determined By Dileucine Internalization Motifs
And/Or By Interaction With Tankyrases
Leucyl-Cystinyl Aminopeptidase
Pregnancy Serum Form: Secreted
During Pregnancy Serum Levels Are Low In The First Trimester
Rise Progressively During The Second And Third Trimester And Decrease Rapidly After Parturition
Alternative Antibody Names Anti-Leucyl-Cystinyl Aminopeptidase antibody
Anti-Cystinyl Aminopeptidase antibody
Anti-Insulin-Regulated Membrane Aminopeptidase antibody
Anti-Insulin-Responsive Aminopeptidase antibody
Anti-Irap antibody
Anti-Oxytocinase antibody
Anti-Otase antibody
Anti-Placental Leucine Aminopeptidase antibody
Anti-P-Lap Cleaved Into - Leucyl-Cystinyl Aminopeptidase - Pregnancy Serum Form antibody
Anti-LNPEP antibody
Anti-OTASE antibody

Information sourced from Uniprot.org

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