• Western blot analysis of extracts of various cell lines, using LPCAT2 antibody (STJ26877) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-LPCAT2 antibody (160-544) (STJ26877)

SKU:
STJ26877

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LPCAT2 (160-544) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LPCAT2
Gene ID: 54947
Uniprot ID: PCAT2_HUMAN
Immunogen Region: 160-544
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-544 of human LPCAT2 (NP_060309.2).
Immunogen Sequence: LPSMVSRNENAQVPLIGRLL RAVQPVLVSRVDPDSRKNTI NEIIKRTTSGGEWPQILVFP EGTCTNRSCLITFKPGAFIP GVPVQPVLLRYPNKLDTVTW TWQGYTFIQLCMLTFCQLFT KVEVEFMPVQVPNDEEKNDP VLFANKVRNLMAEALGIPVT DHTYEDCRLMISAGQLTLPM EAGLVEFTKISRKLKLDWDG VRKHLDEYASIASSSKGGRI GIEEFAKYLKLPVSDVLRQ
Function Exhibits both acyltransferase and acetyltransferase activities. Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Involved in the regulation of lipid droplet number and size.
Protein Name Lysophosphatidylcholine Acyltransferase 2
Lpc Acyltransferase 2
Lpcat-2
Lysopc Acyltransferase 2
1-Acylglycerol-3-Phosphate O-Acyltransferase 11
1-Agp Acyltransferase 11
1-Agpat 11
1-Acylglycerophosphocholine O-Acyltransferase
1-Alkenylglycerophosphocholine O-Acyltransferase
1-Alkylglycerophosphocholine O-Acetyltransferase
Acetyl-Coa -Lyso-Platelet-Activating Factor Acetyltransferase
Acetyl-Coa -Lyso-Paf Acetyltransferase
Lyso-Paf Acetyltransferase
Lysopafat
Acyltransferase-Like 1
Lysophosphatidic Acid Acyltransferase Alpha
Lpaat-Alpha
Database Links Reactome: R-HSA-1482788
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Golgi Apparatus Membrane
Cell Membrane
Lipid Droplet
Alternative Antibody Names Anti-Lysophosphatidylcholine Acyltransferase 2 antibody
Anti-Lpc Acyltransferase 2 antibody
Anti-Lpcat-2 antibody
Anti-Lysopc Acyltransferase 2 antibody
Anti-1-Acylglycerol-3-Phosphate O-Acyltransferase 11 antibody
Anti-1-Agp Acyltransferase 11 antibody
Anti-1-Agpat 11 antibody
Anti-1-Acylglycerophosphocholine O-Acyltransferase antibody
Anti-1-Alkenylglycerophosphocholine O-Acyltransferase antibody
Anti-1-Alkylglycerophosphocholine O-Acetyltransferase antibody
Anti-Acetyl-Coa -Lyso-Platelet-Activating Factor Acetyltransferase antibody
Anti-Acetyl-Coa -Lyso-Paf Acetyltransferase antibody
Anti-Lyso-Paf Acetyltransferase antibody
Anti-Lysopafat antibody
Anti-Acyltransferase-Like 1 antibody
Anti-Lysophosphatidic Acid Acyltransferase Alpha antibody
Anti-Lpaat-Alpha antibody
Anti-LPCAT2 antibody
Anti-AGPAT11 antibody
Anti-AYTL1 antibody

Information sourced from Uniprot.org

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