• Western blot analysis of extracts of various cell lines, using Lipoprotein lipase (LPL) antibody (STJ118691) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using Lipoprotein lipase (LPL) antibody (STJ118691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LPL antibody (124-475) (STJ118691)

SKU:
STJ118691

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LPL (124-475) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LPL
Gene ID: 4023
Uniprot ID: LIPL_HUMAN
Immunogen Region: 124-475
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 124-475 of human Lipoprotein lipase (Lipoprotein lipase (LPL) ) (NP_000228.1).
Immunogen Sequence: SAGYTKLVGQDVARFINWME EEFNYPLDNVHLLGYSLGAH AAGIAGSLTNKKVNRITGLD PAGPNFEYAEAPSRLSPDDA DFVDVLHTFTRGSPGRSIGI QKPVGHVDIYPNGGTFQPGC NIGEAIRVIAERGLGDVDQL VKCSHERSIHLFIDSLLNEE NPSKAYRCSSKEAFEKGLCL SCRKNRCNNLGYEINKVRAK RSSKMYLKTRSQMPYKVFHY QVKIHFSGTESETHTNQAF
Tissue Specificity Detected in blood plasma. Detected in milk (at protein level).
Post Translational Modifications Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.
Function Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.
Protein Name Lipoprotein Lipase
Lpl
Phospholipase A1
Database Links Reactome: R-HSA-381340
Reactome: R-HSA-8963889
Reactome: R-HSA-8963901
Reactome: R-HSA-975634
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Secreted
Extracellular Space
Extracellular Matrix
Newly Synthesized Lpl Binds To Cell Surface Heparan Proteoglycans And Is Then Released By Heparanase
Subsequently
It Becomes Attached To Heparan Proteoglycan On Endothelial Cells
Locates To The Plasma Membrane Of Microvilli Of Hepatocytes With Triglyceride-Rich Lipoproteins (Trl)
Some Of The Bound Lpl Is Then Internalized And Located Inside Non-Coated Endocytic Vesicles
Alternative Antibody Names Anti-Lipoprotein Lipase antibody
Anti-Lpl antibody
Anti-Phospholipase A1 antibody
Anti-LPL antibody
Anti-LIPD antibody

Information sourced from Uniprot.org

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