• Western blot analysis of extracts of various cell lines, using LYPLA1 antibody (STJ24436) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded Human liver cancer using LYPLA1 rabbit polyclonal antibody (STJ24436) at dilution of 1:100 (40x lens).

Anti-LYPLA1 antibody (1-230) (STJ24436)

SKU:
STJ24436

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LYPLA1 (1-230) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LYPLA1
Gene ID: 10434
Uniprot ID: LYPA1_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human LYPLA1 (NP_006321.1).
Immunogen Sequence: MCGNNMSTPLPAIVPAARKA TAAVIFLHGLGDTGHGWAEA FAGIRSSHIKYICPHAPVRP VTLNMNVAMPSWFDIIGLSP DSQEDESGIKQAAENIKALI DQEVKNGIPSNRIILGGFSQ GGALSLYTALTTQQKLAGVT ALSCWLPLRASFPQGPIGGA NRDISILQCHGDCDPLVPLM FGSLTVEKLKTLVNPANVTF KTYEGMMHSSCQQEMMDVKQ FIDKLLPPID
Tissue Specificity Platelets.
Function Acts as a acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Could also depalmitoylate ADRB2. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA.
Protein Name Acyl-Protein Thioesterase 1
Apt-1
Hapt1
Lysophospholipase 1
Lysophospholipase I
Lpl-I
Lysopla I
Palmitoyl-Protein Hydrolase
Database Links Reactome: R-HSA-203615
Reactome: R-HSA-9648002
Cellular Localisation Cytoplasm
Cell Membrane
Nucleus Membrane
Endoplasmic Reticulum
Shows Predominantly A Cytoplasmic Localization With A Weak Expression In The Cell Membrane
Nuclear Membrane And Endoplasmic Reticulum
Alternative Antibody Names Anti-Acyl-Protein Thioesterase 1 antibody
Anti-Apt-1 antibody
Anti-Hapt1 antibody
Anti-Lysophospholipase 1 antibody
Anti-Lysophospholipase I antibody
Anti-Lpl-I antibody
Anti-Lysopla I antibody
Anti-Palmitoyl-Protein Hydrolase antibody
Anti-LYPLA1 antibody
Anti-APT1 antibody
Anti-LPL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance