• Western blot analysis of extracts of various cell lines, using MAN1B1 antibody (STJ115729) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAN1B1 antibody (1-290) (STJ115729)

SKU:
STJ115729

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAN1B1 (1-290) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAN1B1
Gene ID: 11253
Uniprot ID: MA1B1_HUMAN
Immunogen Region: 1-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human MAN1B1 (NP_057303.2).
Immunogen Sequence: MAACEGRRSGALGSSQSDFL TPPVGGAPWAVATTVVMYPP PPPPPHRDFISVTLSFGENY DNSKSWRRRSCWRKWKQLSR LQRNMILFLLAFLLFCGLLF YINLADHWKALAFRLEEEQK MRPEIAGLKPANPPVLPAPQ KADTDPENLPEISSQKTQRH IQRGPPHLQIRPPSQDLKDG TQEEATKRQEAPVDPRPEGD PQRTVISWRGAVIEPEQGTE LPSRRAEVPTKPPLPPART
Tissue Specificity Widely expressed.
Function Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2).
Protein Name Endoplasmic Reticulum Mannosyl-Oligosaccharide 1 -2-Alpha-Mannosidase
Er Alpha-1 -2-Mannosidase
Er Mannosidase 1
Erman1
Man9glcnac2-Specific-Processing Alpha-Mannosidase
Mannosidase Alpha Class 1b Member 1
Database Links Reactome: R-HSA-4793950
Reactome: R-HSA-901032
Reactome: R-HSA-9694548
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Endoplasmic Reticulum Mannosyl-Oligosaccharide 1 -2-Alpha-Mannosidase antibody
Anti-Er Alpha-1 -2-Mannosidase antibody
Anti-Er Mannosidase 1 antibody
Anti-Erman1 antibody
Anti-Man9glcnac2-Specific-Processing Alpha-Mannosidase antibody
Anti-Mannosidase Alpha Class 1b Member 1 antibody
Anti-MAN1B1 antibody
Anti-UNQ747 antibody
Anti-PRO1477 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance