• Western blot analysis of extracts of HeLa cells, using MASP1 antibody (STJ27660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MASP1 antibody (20-300) (STJ27660)

SKU:
STJ27660

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MASP1 (20-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MASP1
Gene ID: 5648
Uniprot ID: MASP1_HUMAN
Immunogen Region: 20-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 20-300 of human MASP1 (NP_001870.3).
Immunogen Sequence: HTVELNNMFGQIQSPGYPDS YPSDSEVTWNITVPDGFRIK LYFMHFNLESSYLCEYDYVK VETEDQVLATFCGRETTDTE QTPGQEVVLSPGSFMSITFR SDFSNEERFTGFDAHYMAVD VDECKEREDEELSCDHYCHN YIGGYYCSCRFGYILHTDNR TCRVECSDNLFTQRTGVITS PDFPNPYPKSSECLYTIELE EGFMVNLQFEDIFDIEDHPE VPCPYDYIKIKVGPKVLGP
Tissue Specificity Protein of the plasma which is primarily expressed by liver.
Post Translational Modifications The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. N-glycosylated. Some N-linked glycan are of the complex-type. Autoproteolytic processing of the proenzyme produces the active enzyme composed on the heavy and the light chain held together by a disulfide bond. Isoform 1 but not isoform 2 is activated through autoproteolytic processing.
Function Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. Also plays a role in development.
Protein Name Mannan-Binding Lectin Serine Protease 1
Complement Factor Masp-3
Complement-Activating Component Of Ra-Reactive Factor
Mannose-Binding Lectin-Associated Serine Protease 1
Masp-1
Mannose-Binding Protein-Associated Serine Protease
Ra-Reactive Factor Serine Protease P100
Rarf
Serine Protease 5 Cleaved Into - Mannan-Binding Lectin Serine Protease 1 Heavy Chain - Mannan-Binding Lectin Serine Protease 1 Light Chain
Database Links Reactome: R-HSA-166662
Reactome: R-HSA-166663
Reactome: R-HSA-2855086
Reactome: R-HSA-3000480
Reactome: R-HSA-9705671
Cellular Localisation Secreted
Alternative Antibody Names Anti-Mannan-Binding Lectin Serine Protease 1 antibody
Anti-Complement Factor Masp-3 antibody
Anti-Complement-Activating Component Of Ra-Reactive Factor antibody
Anti-Mannose-Binding Lectin-Associated Serine Protease 1 antibody
Anti-Masp-1 antibody
Anti-Mannose-Binding Protein-Associated Serine Protease antibody
Anti-Ra-Reactive Factor Serine Protease P100 antibody
Anti-Rarf antibody
Anti-Serine Protease 5 Cleaved Into - Mannan-Binding Lectin Serine Protease 1 Heavy Chain - Mannan-Binding Lectin Serine Protease 1 Light Chain antibody
Anti-MASP1 antibody
Anti-CRARF antibody
Anti-CRARF1 antibody
Anti-PRSS5 antibody

Information sourced from Uniprot.org

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