• Western blot analysis of extracts of various cell lines, using MDH2 antibody (STJ28219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded human liver damage using MDH2 Antibody (STJ28219) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver damage using MDH2 Antibody (STJ28219) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using MDH2 Antibody (STJ28219) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using MDH2 Polyclonal Antibody (STJ28219) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using MDH2 Polyclonal Antibody (STJ28219) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MDH2 antibody (59-338) (STJ28219)

SKU:
STJ28219

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MDH2 (59-338) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MDH2
Gene ID: 4191
Uniprot ID: MDHM_HUMAN
Immunogen Region: 59-338
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 59-338 of human MDH2 (NP_005909.2).
Immunogen Sequence: AHTPGVAADLSHIETKAAVK GYLGPEQLPDCLKGCDVVVI PAGVPRKPGMTRDDLFNTNA TIVATLTAACAQHCPEAMIC VIANPVNSTIPITAEVFKKH GVYNPNKIFGVTTLDIVRAN TFVAELKGLDPARVNVPVIG GHAGKTIIPLISQCTPKVDF PQDQLTALTGRIQEAGTEVV KAKAGAGSATLSMAYAGARF VFSLVDAMNGKEGVVECSFV KSQETECTYFSTPLLLGKK
Post Translational Modifications Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%.
Protein Name Malate Dehydrogenase - Mitochondrial
Database Links Reactome: R-HSA-70263
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Malate Dehydrogenase - Mitochondrial antibody
Anti-MDH2 antibody

Information sourced from Uniprot.org

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