| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ME1 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ME1 |
| Gene ID: |
4199 |
| Uniprot ID: |
MAOX_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ME1 (NP_002386.1). |
| Immunogen Sequence: |
MEPEAPRRRHTHQRGYLLTR NPHLNKDLAFTLEERQQLNI HGLLPPSFNSQEIQVLRVVK NFEHLNSDFDRYLLLMDLQD RNEKLFYRVLTSDIEKFMPI VYTPTVGLACQQYSLVFRKP RGLFITIHDRGHIASVLNAW PEDVIKAIVVTDGERILGLG DLGCNGMGIPVGKLALYTAC GGMNPQECLPVILDVGTENE ELLKDPLYIGLRQRRVRGSE YDDFLDEFMEAVSSKYGMN |
| Tissue Specificity | Expressed in all tissues tested including liver, placenta and white adipose tissue. |
| Function | Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP(+) and divalent metal ions, and decarboxylation of oxaloacetate. |
| Protein Name | Nadp-Dependent Malic EnzymeNadp-MeMalic Enzyme 1 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-70268Reactome: R-HSA-9818025 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Nadp-Dependent Malic Enzyme antibodyAnti-Nadp-Me antibodyAnti-Malic Enzyme 1 antibodyAnti-ME1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
