Anti-Myosin VI antibody

[STJ96437] Download PDF Print Data Sheet

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Product name Anti-Myosin VI antibody
Short Description Rabbit polyclonal against Myosin VI
Description Myosin VI is a protein encoded by the MYO6 gene which is approximately 149, 6 kDa. Myosin VI is localised to the Golgi apparatus, nucleus and cytoplasm. It is involved in the PAK pathway, gap junction trafficking, actin nucleation and the RhoGDI pathway. It is a reverse-direction motor protein with ATPase activity that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. Myosin VI is expressed in most tissues including heart, brain, placenta, pancreas and spleen. Mutations in the MYO6 gene may result in deafness. STJ96437 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Myosin VI protein.
Applications ELISA, IHC-p, WB
Dilution range WB 1:500-1:2000
IHC-p 1:100-300
ELISA 1:5000
Specificity Myosin VI Polyclonal Antibody detects endogenous levels of Myosin VI protein.
Protein Name Unconventional Myosin-Vi
Unconventional Myosin-6
Immunogen Synthesized peptide derived from human Myosin VI
Immunogen Region 40-120 aa, N-terminal
Storage Instruction Store at-20°C, and avoid repeat freeze-thaw cycles.
Note FOR RESEARCH USE ONLY (RUO).
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 4646
Gene Symbol MYO6
Molecular Weight 150 kDa
Database Links HGNC:7605
OMIM:600970
Alternative Names Anti-Unconventional Myosin-Vi antibody
Anti-Unconventional Myosin-6 antibody
Anti-MYO6 KIAA0389 antibody
Function Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. As part of the DISP complex, may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. May play a role in the extension and network organization of neurites. Required for structural integrity of inner ear hair cells. Modulates RNA polymerase II-dependent transcription.
Post-translational Modifications Phosphorylation in the motor domain, induced by EGF, results in translocation of MYO6 from the cell surface to membrane ruffles and affects F-actin dynamics. Phosphorylated in vitro by p21-activated kinase (PAK).
Cellular Localization Golgi Apparatus
Trans-Golgi Network Membrane
Peripheral Membrane Protein
Nucleus
Cytoplasm
Perinuclear Region
Membrane
Clathrin-Coated Pit
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Cell Projection
Filopodium
Ruffle Membrane
Microvillus
Cytosol
Also Present In Endocyctic Vesicles
Translocates From Membrane Ruffles
Endocytic Vesicles And Cytoplasm To Golgi Apparatus
Perinuclear Membrane And Nucleus Through Induction By P53 And P53-Induced Dna Damage
Recruited Into Membrane Ruffles From Cell Surface By Egf-Stimulation
Colocalizes With Dab2 In Clathrin-Coated Pits/Vesicles
Colocalizes With Optn At The Golgi Complex And In Vesicular Structures Close To The Plasma Membrane (By Similarity)
Isoform 3: Cytoplasmic Vesicle
Clathrin-Coated Vesicle Membrane
Isoform 4: Cytoplasmic Vesicle
Tissue Specificity Expressed in most tissues examined including heart, brain, placenta, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in brain, pancreas, testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2, containing the small insert, and isoform 4, containing neither insert, are expressed in unpolarized epithelial cells.
Swiss-Prot Key MYO6_HUMAN
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