• Western blot analysis of extracts of various cell lines, using NAE1 antibody (STJ24676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NAE1 antibody (1-220) (STJ24676)

SKU:
STJ24676

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NAE1 (1-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NAE1
Gene ID: 8883
Uniprot ID: ULA1_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NAE1 (NP_003896.1).
Immunogen Sequence: MAQLGKLLKEQKYDRQLRLW GDHGQEALESAHVCLINATA TGTEILKNLVLPGIGSFTII DGNQVSGEDAGNNFFLQRSS IGKNRAEAAMEFLQELNSDV SGSFVEESPENLLDNDPSFF CRFTVVVATQLPESTSLRLA DVLWNSQIPLLICRTYGLVG YMRIIIKEHPVIESHPDNAL EDLRLDKPFPELREHFQSYD LDHMEKKDHSHTPWIVIIAK
Tissue Specificity Ubiquitous in fetal tissues. Expressed throughout the adult brain.
Post Translational Modifications Ubiquitinated by TRIP12, leading to its degradation by the proteasome.
Function Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development.
Protein Name Nedd8-Activating Enzyme E1 Regulatory Subunit
Amyloid Beta Precursor Protein-Binding Protein 1 - 59 Kda
App-Bp1
Amyloid Protein-Binding Protein 1
Proto-Oncogene Protein 1
Database Links Reactome: R-HSA-8951664
Cellular Localisation Cell Membrane
Colocalizes With App In Lipid Rafts
Alternative Antibody Names Anti-Nedd8-Activating Enzyme E1 Regulatory Subunit antibody
Anti-Amyloid Beta Precursor Protein-Binding Protein 1 - 59 Kda antibody
Anti-App-Bp1 antibody
Anti-Amyloid Protein-Binding Protein 1 antibody
Anti-Proto-Oncogene Protein 1 antibody
Anti-NAE1 antibody
Anti-APPBP1 antibody
Anti-HPP1 antibody

Information sourced from Uniprot.org

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