• Western blot analysis of extracts of various cell lines, using NCOA5 antibody (STJ116208) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NCOA5 antibody (240-380) (STJ116208)

SKU:
STJ116208

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCOA5 (240-380) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCOA5
Gene ID: 57727
Uniprot ID: NCOA5_HUMAN
Immunogen Region: 240-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human NCOA5 (NP_066018.1).
Immunogen Sequence: QALEDVSRGGSPFAIVITQQ HQIHRSCTVNIMFGTPQEHR NMPQADAMVLVARNYERYKN ECREKEREEIARQAAKMADE AILQERERGGPEEGVRGGHP PAIQSLINLLADNRYLTAEE TDKIINYLRERKERLMRSST D
Tissue Specificity Widely expressed.
Function Nuclear receptor coregulator that can have both coactivator and corepressor functions. Interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF-2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. Involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC in response to 17-beta-estradiol (E2).
Protein Name Nuclear Receptor Coactivator 5
Ncoa-5
Coactivator Independent Of Af-2
Cia
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Receptor Coactivator 5 antibody
Anti-Ncoa-5 antibody
Anti-Coactivator Independent Of Af-2 antibody
Anti-Cia antibody
Anti-NCOA5 antibody
Anti-KIAA1637 antibody

Information sourced from Uniprot.org

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