• Western blot analysis of the lysates from HepG2 cells using NDUFA4 antibody.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from HepG2 and RAW264.7 cells, using NDUFA4 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using NDUFA4 Polyclonal Antibody

Anti-NDUFA4 antibody (32-81) (STJ94373)

SKU:
STJ94373

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cytochrome c oxidase subunit NDUFA4 (32-81) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFA4
Gene ID: 4697
Uniprot ID: NDUA4_HUMAN
Immunogen Region: 32-81
Specificity: NDUFA4 Polyclonal Antibody detects endogenous levels of NDUFA4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human NDUFA4. AA range:32-81
Function Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. NDUFA4 is required for complex IV maintenance.
Protein Name Cytochrome C Oxidase Subunit Ndufa4
Complex I-Mlrq
Ci-Mlrq
Nadh-Ubiquinone Oxidoreductase Mlrq Subunit
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome C Oxidase Subunit Ndufa4 antibody
Anti-Complex I-Mlrq antibody
Anti-Ci-Mlrq antibody
Anti-Nadh-Ubiquinone Oxidoreductase Mlrq Subunit antibody
Anti-NDUFA4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance