• Immunohistochemistry analysis of paraffin-embedded rat kidney using NDUFS4 Rabbit polyclonal antibody (STJ28473) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat brain, using NDUFS4 antibody (STJ28473) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NDUFS4 antibody (1-175) (STJ28473)

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STJ28473

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFS4 (1-175) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS4
Gene ID: 4724
Uniprot ID: NDUS4_HUMAN
Immunogen Region: 1-175
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human NDUFS4 (NP_002486.1).
Immunogen Sequence: MAAVSMSVVLRQTLWRRRAV AVAALSVSRVPTRSLRTSTW RLAQDQTQDTQLITVDEKLD ITTLTGVPEEHIKTRKVRIF VPARNNMQSGVNNTKKWKME FDTRERWENPLMGWASTADP LSNMVLTFSTKEDAVSFAEK NGWSYDIEERKVPKPKSKSY GANFSWNKRTRVSTK
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 4 - Mitochondrial
Complex I-18 Kda
Ci-18 Kda
Complex I-Aqdq
Ci-Aqdq
Nadh-Ubiquinone Oxidoreductase 18 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
The Interaction With Bcap31 Mediates Mitochondria Localization
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 4 - Mitochondrial antibody
Anti-Complex I-18 Kda antibody
Anti-Ci-18 Kda antibody
Anti-Complex I-Aqdq antibody
Anti-Ci-Aqdq antibody
Anti-Nadh-Ubiquinone Oxidoreductase 18 Kda Subunit antibody
Anti-NDUFS4 antibody

Information sourced from Uniprot.org

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