• Immunohistochemistry analysis of NFIB in paraffin-embedded rat lung using NFIB Rabbit polyclonal antibody (STJ117489) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using NFIB Rabbit polyclonal antibody (STJ117489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-NFIB antibody (C-Term) (STJ117489)

SKU:
STJ117489

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFIB (C-Term) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFIB
Gene ID: 4781
Uniprot ID: NFIB_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human NFIB (NP_005587.2).
Immunogen Sequence: SRTWHERDQDMSSPTTMKKP EKPLFSSASPQDSSPRLSTF PQHHHPGIPGVAHSVISTRT PPPPSPLPFPTQAILPPAPS SYFSHPTIRYPPHLNPQDTL KNYVPSYDPSSPQTSQSWYL G
Function Transcriptional activator of GFAP, essential for proper brain development. Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Protein Name Nuclear Factor 1 B-Type
Nf1-B
Nuclear Factor 1/B
Ccaat-Box-Binding Transcription Factor
Ctf
Nuclear Factor I/B
Nf-I/B
Nfi-B
Tggca-Binding Protein
Database Links Reactome: R-HSA-73980
Reactome: R-HSA-749476
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Factor 1 B-Type antibody
Anti-Nf1-B antibody
Anti-Nuclear Factor 1/B antibody
Anti-Ccaat-Box-Binding Transcription Factor antibody
Anti-Ctf antibody
Anti-Nuclear Factor I/B antibody
Anti-Nf-I/B antibody
Anti-Nfi-B antibody
Anti-Tggca-Binding Protein antibody
Anti-NFIB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance