| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NFKBIZ (1-220) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NFKBIZ |
| Gene ID: |
64332 |
| Uniprot ID: |
IKBZ_HUMAN |
| Immunogen Region: |
1-220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NFKBIZ (NP_113607.1). |
| Immunogen Sequence: |
MIVDKLLDDSRGGEGLRDAA GGCGLMTSPLNLSYFYGASP PAAAPGACDASCSVLGPSAP GSPGSDSSDFSSASSVSSCG AVESRSRGGARAERQPVEPH MGVGRQQRGPFQGVRVKNSV KELLLHIRSHKQKASGQAVD DFKTQGVNIEQFRELKNTVS YSGKRKGPDSLSDGPACKRP ALLHSQFLTPPQTPTPGESM EDVHLNEPKQESSADLLQNI |
| Tissue Specificity | Expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. |
| Function | Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF-kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. Involved in the induction of T helper 17 cells (Th17) differentiation upon recognition of antigen by T cell antigen receptor (TCR). |
| Protein Name | Nf-Kappa-B Inhibitor ZetaI-Kappa-B-ZetaIkb-ZetaIkappabzetaIl-1 Inducible Nuclear Ankyrin-Repeat ProteinInapMolecule Possessing Ankyrin Repeats Induced By LipopolysaccharideMail |
| Cellular Localisation | NucleusAggregated In Dot-Like StructuresColocalizes With Ncor2 |
| Alternative Antibody Names | Anti-Nf-Kappa-B Inhibitor Zeta antibodyAnti-I-Kappa-B-Zeta antibodyAnti-Ikb-Zeta antibodyAnti-Ikappabzeta antibodyAnti-Il-1 Inducible Nuclear Ankyrin-Repeat Protein antibodyAnti-Inap antibodyAnti-Molecule Possessing Ankyrin Repeats Induced By Lipopolysaccharide antibodyAnti-Mail antibodyAnti-NFKBIZ antibodyAnti-IKBZ antibodyAnti-INAP antibodyAnti-MAIL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Emerin/Emerin/EMD knockout (KO) 293T cells, using [KO Validated] Emerin/EMD Rabbit polyclonal antibody (STJ119989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98774/382822/STJ119989_1__22320.1713151321.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Emerin/Emerin/EMD knockout (KO) 293T cells, using [KO Validated] Emerin/EMD Rabbit polyclonal antibody (STJ119989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")