| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NISCH (500-700) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NISCH |
| Gene ID: |
11188 |
| Uniprot ID: |
NISCH_HUMAN |
| Immunogen Region: |
500-700 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human NISCH (NP_009115.2). |
| Immunogen Sequence: |
SLPQPILSNQGIMFVQEEAL ASSLSSTDSLTPEHQPIAQG CSDSLESIPAGQAASDDLRD VPGAVGGASPEHAEPEVQVV PGSGQIIFLPFTCIGYTATN QDFIQRLSTLIRQAIERQLP AWIEAANQREEGQGEQGEEE DEEEEEEEDVAENRYFEMGP PDVEEEEGGGQGEEEEEEEE DEEAEEERLALEWALGADED F |
| Tissue Specificity | Isoform 1, isoform 3 and isoform 4 are expressed in brain. Isoform 1 is expressed in endocrine tissues. |
| Function | Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures. |
| Protein Name | NischarinImidazoline Receptor 1I-1Ir1Imidazoline Receptor Antisera-Selected ProteinHirasImidazoline-1 ReceptorI1rImidazoline-1 Receptor Candidate ProteinI-1 Receptor Candidate ProteinI1r Candidate Protein |
| Database Links | Reactome: R-HSA-9013149Reactome: R-HSA-9696264Reactome: R-HSA-9696270 |
| Cellular Localisation | Cell MembraneCytoplasmEarly EndosomeRecycling EndosomeEnriched In The Early/Sorting And Recycling EndosomesColocalized In Early/Sorting Endosomes With Eea1 And Snx2 And In Recycling Endosomes With Transferrin ReceptorDetected In The Perinuclear Region Partially Associated With Punctate StructuresColocalizes With Pak1 In CytoplasmVesicular Structures In The Perinuclear Area And Membrane RufflesColocalizes With Rac1 In The Cytoplasm And Vesicles StructuresColocalized With Mapk1 And Mapk3 In Rvlm Neurons |
| Alternative Antibody Names | Anti-Nischarin antibodyAnti-Imidazoline Receptor 1 antibodyAnti-I-1 antibodyAnti-Ir1 antibodyAnti-Imidazoline Receptor Antisera-Selected Protein antibodyAnti-Hiras antibodyAnti-Imidazoline-1 Receptor antibodyAnti-I1r antibodyAnti-Imidazoline-1 Receptor Candidate Protein antibodyAnti-I-1 Receptor Candidate Protein antibodyAnti-I1r Candidate Protein antibodyAnti-NISCH antibodyAnti-IRAS antibodyAnti-KIAA0975 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
