• Western blot analysis of extracts of various cell lines, using NR2C1 antibody (STJ28758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of MCF7 cells using NR2C1 antibody (STJ28758).

Anti-NR2C1 antibody (1-240) (STJ28758)

SKU:
STJ28758

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NR2C1 (1-240) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NR2C1
Gene ID: 7181
Uniprot ID: NR2C1_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NR2C1 (NP_003288.2).
Immunogen Sequence: MATIEEIAHQIIEQQMGEIV TEQQTGQKIQIVTALDHNTQ GKQFILTNHDGSTPSKVILA RQDSTPGKVFLTTPDAAGVN QLFFTTPDLSAQHLQLLTDN SPDQGPNKVFDLCVVCGDKA SGRHYGAVTCEGCKGFFKRS IRKNLVYSCRGSKDCIINKH HRNRCQYCRLQRCIAFGMKQ DSVQCERKPIEVSREKSSNC AASTEKIYIRKDLRSPLTAT PTFVTDSESTRSTGLLDSG
Post Translational Modifications Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression. Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-222, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-581 by PKC is important for protein stability and function as activator of RARB.
Function Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation.
Protein Name Nuclear Receptor Subfamily 2 Group C Member 1
Orphan Nuclear Receptor Tr2
Testicular Receptor 2
Database Links Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Cellular Localisation Nucleus
Pml Body
Recruited By Hdac3
After All-Trans Retinoic Acid Stimulated Mapk1-Mediated Thr-223 Phosphorylation
To Pml Bodies For Subsequent Sumoylation
Alternative Antibody Names Anti-Nuclear Receptor Subfamily 2 Group C Member 1 antibody
Anti-Orphan Nuclear Receptor Tr2 antibody
Anti-Testicular Receptor 2 antibody
Anti-NR2C1 antibody
Anti-TR2 antibody

Information sourced from Uniprot.org

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