• Western blot analysis of extracts of MCF-7 cells, using NRG1 antibody (STJ114893). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-NRG1 antibody (1-210) (STJ114893)

SKU:
STJ114893

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NRG1 (1-210) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRG1
Gene ID: 3084
Uniprot ID: NRG1_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human NRG1 (NP_039252.2).
Immunogen Sequence: MSERKEGRGKGKGKKKERGS GKKPESAAGSQSPALPPRLK EMKSQESAAGSKLVLRCETS SEYSSLRFKWFKNGNELNRK NKPQNIKIQKKPGKSELRIN KASLADSGEYMCKVISKLGN DSASANITIVESNEIITGMP ASTEGAYVSSESPIRISVST EGANTSSSTSTSTTGTSHLV KCAEKEKTFCVNGGECFMVK DLSNPSRYLC
Tissue Specificity Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells.in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific.
Post Translational Modifications Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage.
Function Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells.inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction.stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells.stimulating Schwann cell proliferation.implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4. Binds to ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons.
Protein Name Pro-Neuregulin-1 - Membrane-Bound Isoform
Pro-Nrg1 Cleaved Into - Neuregulin-1
Acetylcholine Receptor-Inducing Activity
Aria
Breast Cancer Cell Differentiation Factor P45
Glial Growth Factor
Heregulin
Hrg
Neu Differentiation Factor
Sensory And Motor Neuron-Derived Factor
Database Links Reactome: R-HSA-1227986 Q02297-10
Reactome: R-HSA-1236394 Q02297-10
Reactome: R-HSA-1250196 Q02297-10
Reactome: R-HSA-1250342
Reactome: R-HSA-1250347
Reactome: R-HSA-1251985
Reactome: R-HSA-1257604 Q02297-10
Reactome: R-HSA-1306955 Q02297-10
Reactome: R-HSA-1358803 Q02297-10
Reactome: R-HSA-1963640
Reactome: R-HSA-1963642 Q02297-10
Reactome: R-HSA-2219530 Q02297-10
Reactome: R-HSA-5673001 Q02297-10
Reactome: R-HSA-6785631 Q02297-10
Reactome: R-HSA-6811558 Q02297-10
Reactome: R-HSA-8847993 Q02297-10
Reactome: R-HSA-8863795 Q02297-10
Reactome: R-HSA-9620244
Reactome: R-HSA-9664565 Q02297-10
Reactome: R-HSA-9665686 Q02297-10
Cellular Localisation Pro-Neuregulin-1
Membrane-Bound Isoform: Cell Membrane
Single-Pass Type I Membrane Protein
Does Not Seem To Be Active
Neuregulin-1: Secreted
Isoform 8: Nucleus
May Be Nuclear
Isoform 9: Secreted
Has A Signal Peptide
Isoform 10: Membrane
May Possess An Internal Uncleaved Signal Sequence
Alternative Antibody Names Anti-Pro-Neuregulin-1 - Membrane-Bound Isoform antibody
Anti-Pro-Nrg1 Cleaved Into - Neuregulin-1 antibody
Anti-Acetylcholine Receptor-Inducing Activity antibody
Anti-Aria antibody
Anti-Breast Cancer Cell Differentiation Factor P45 antibody
Anti-Glial Growth Factor antibody
Anti-Heregulin antibody
Anti-Hrg antibody
Anti-Neu Differentiation Factor antibody
Anti-Sensory And Motor Neuron-Derived Factor antibody
Anti-NRG1 antibody
Anti-GGF antibody
Anti-HGL antibody
Anti-HRGA antibody
Anti-NDF antibody
Anti-SMDF antibody

Information sourced from Uniprot.org

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