• Western blot analysis of extracts of rat liver, using NUAK2 antibody (STJ24842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NUAK2 antibody (479-628) (STJ24842)

SKU:
STJ24842

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUAK2 (479-628) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUAK2
Gene ID: 81788
Uniprot ID: NUAK2_HUMAN
Immunogen Region: 479-628
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 479-628 of human NUAK2 (NP_112214.3).
Immunogen Sequence: VSGDPKEQKPPQASGLLLHR KGILKLNGKFSQTALELAAP TTFGSLDELAPPRPLARASR PSGAVSEDSILSSESFDQLD LPERLPEPPLRGCVSVDNLT GLEEPPSEGPGSCLRRWRQD PLGDSCFSLTDCQEVTATYR QALRVCSKLT
Post Translational Modifications Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Autophosphorylation is also possible at Thr-208.
Function Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Phosphorylates LATS1 and LATS2. Plays a key role in neural tube closure during embryonic development through LATS2 phosphorylation and regulation of the nuclear localization of YAP1 a critical downstream regulatory target in the Hippo signaling pathway.
Protein Name Nuak Family Snf1-Like Kinase 2
Omphalocele Kinase 2
Snf1/Amp Kinase-Related Kinase
Snark
Alternative Antibody Names Anti-Nuak Family Snf1-Like Kinase 2 antibody
Anti-Omphalocele Kinase 2 antibody
Anti-Snf1/Amp Kinase-Related Kinase antibody
Anti-Snark antibody
Anti-NUAK2 antibody
Anti-OMPHK2 antibody
Anti-SNARK antibody

Information sourced from Uniprot.org

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