• Western blot analysis of extracts of various cell lines, using NUDT21 antibody (STJ24845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using NUDT21 rabbit polyclonal antibody (STJ24845) at dilution of 1:100 (40x lens).

Anti-NUDT21 antibody (1-227) (STJ24845)

SKU:
STJ24845

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUDT21 (1-227) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUDT21
Gene ID: 11051
Uniprot ID: CPSF5_HUMAN
Immunogen Region: 1-227
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human NUDT21 (NP_008937.1).
Immunogen Sequence: MSVVPPNRSQTGWPRGVTQF GNKYIQQTKPLTLERTINLY PLTNYTFGTKEPLYEKDSSV AARFQRMREEFDKIGMRRTV EGVLIVHEHRLPHVLLLQLG TTFFKLPGGELNPGEDEVEG LKRLMTEILGRQDGVLQDWV IDDCIGNWWRPNFEPPQYPY IPAHITKPKEHKKLFLVQLQ EKALFAVPKNYKLVAAPLFE LYDNAPGYGPIISSLPQLLS RFNFIYN
Tissue Specificity Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post Translational Modifications Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 and SIRT2.
Function Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function. Binds to chromatin. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides.
Protein Name Cleavage And Polyadenylation Specificity Factor Subunit 5
Cleavage And Polyadenylation Specificity Factor 25 Kda Subunit
Cpsf 25 Kda Subunit
Cleavage Factor Im Complex 25 Kda Subunit
Cfim25
Nucleoside Diphosphate-Linked Moiety X Motif 21
Nudix Motif 21
Nudix Hydrolase 21
Pre-Mrna Cleavage Factor Im 68 Kda Subunit
Database Links Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And The Cytoplasm In A Transcription- And Xpo1/Crm1-Independent Manner
Most Probably In Complex With The Cleavage Factor Im Complex (Cfim)
In Punctate Subnuclear Structures Localized Adjacent To Nuclear Speckles
Called Paraspeckles
Alternative Antibody Names Anti-Cleavage And Polyadenylation Specificity Factor Subunit 5 antibody
Anti-Cleavage And Polyadenylation Specificity Factor 25 Kda Subunit antibody
Anti-Cpsf 25 Kda Subunit antibody
Anti-Cleavage Factor Im Complex 25 Kda Subunit antibody
Anti-Cfim25 antibody
Anti-Nucleoside Diphosphate-Linked Moiety X Motif 21 antibody
Anti-Nudix Motif 21 antibody
Anti-Nudix Hydrolase 21 antibody
Anti-Pre-Mrna Cleavage Factor Im 68 Kda Subunit antibody
Anti-NUDT21 antibody
Anti-CFIM25 antibody
Anti-CPSF25 antibody
Anti-CPSF5 antibody

Information sourced from Uniprot.org

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