• Western blot analysis of extracts of various cell lines, using NUP133 antibody (STJ111432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using NUP133 antibody (STJ111432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NUP133 antibody (730-930) (STJ111432)

SKU:
STJ111432

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUP133 (730-930) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUP133
Gene ID: 55746
Uniprot ID: NU133_HUMAN
Immunogen Region: 730-930
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 730-930 of human NUP133 (NP_060700.2).
Immunogen Sequence: NILKDMLQAASHYRQNRNSL YRREESLEKEPEYVPWTATS GPGGIRTVIIRQHEIVLKVA YPQADSNLRNIVTEQLVALI DCFLDGYVSQLKSVDKSSNR ERYDNLEMEYLQKRSDLLSP LLSLGQYLWAASLAEKYCDF DILVQMCEQTDNQSRLQRYM TQFADQNFSDFLFRWYLEKG KRGKLLSQPISQHGQLANFL Q
Tissue Specificity Widely expressed in fetal and adult tissues. Expressed in the brain and kidney.
Function Involved in poly(A)+ RNA transport. Involved in nephrogenesis.
Protein Name Nuclear Pore Complex Protein Nup133
133 Kda Nucleoporin
Nucleoporin Nup133
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-141444
Reactome: R-HSA-159227
Reactome: R-HSA-159230
Reactome: R-HSA-159231
Reactome: R-HSA-159236
Reactome: R-HSA-165054
Reactome: R-HSA-168271
Reactome: R-HSA-168276
Reactome: R-HSA-168325
Reactome: R-HSA-168333
Reactome: R-HSA-170822
Reactome: R-HSA-180746
Reactome: R-HSA-180910
Reactome: R-HSA-191859
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-3108214
Reactome: R-HSA-3232142
Reactome: R-HSA-3301854
Reactome: R-HSA-3371453
Reactome: R-HSA-4085377
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-5578749
Reactome: R-HSA-5619107
Reactome: R-HSA-5663220
Reactome: R-HSA-6784531
Reactome: R-HSA-68877
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9615933
Reactome: R-HSA-9648025
Reactome: R-HSA-9705671
Cellular Localisation Nucleus
Nuclear Pore Complex
Chromosome
Centromere
Kinetochore
Located On Both The Cytoplasmic And Nuclear Sides Of The Nuclear Pore
During Mitosis
Localizes To The Kinetochores
Alternative Antibody Names Anti-Nuclear Pore Complex Protein Nup133 antibody
Anti-133 Kda Nucleoporin antibody
Anti-Nucleoporin Nup133 antibody
Anti-NUP133 antibody

Information sourced from Uniprot.org

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