• Western blot analysis of K562 using Otubain-1 antibody.. Secondary antibody was diluted at 1:20000

Anti-OTUB1 antibody (20-100 aa) (STJ97632)

SKU:
STJ97632

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ubiquitin thioesterase OTUB1 (20-100 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OTUB1
Gene ID: 55611
Uniprot ID: OTUB1_HUMAN
Immunogen Region: 20-100 aa
Specificity: Otubain-1 Polyclonal Antibody detects endogenous levels of Otubain-1 protein.
Immunogen: Synthesized peptide derived from Otubain-1 at the amino acid range 20-100
Post Translational Modifications Phosphorylation at Tyr-26 by SRC and SRMS promotes deubiquitination of RPTOR via a non-catalytic process.
Function Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin. Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain. Acts as a regulator of mTORC1 and mTORC2 complexes. When phosphorylated at Tyr-26, acts as an activator of the mTORC1 complex by mediating deubiquitination of RPTOR via a non-catalytic process: acts by binding and inhibiting the activity of the ubiquitin-conjugating enzyme E2 (UBE2D1/UBCH5A, UBE2W/UBC16 and UBE2N/UBC13), thereby preventing ubiquitination of RPTOR. Can also act as an inhibitor of the mTORC1 and mTORC2 complexes in response to amino acids by mediating non-catalytic deubiquitination of DEPTOR.
Protein Name Ubiquitin Thioesterase Otub1
Deubiquitinating Enzyme Otub1
Otu Domain-Containing Ubiquitin Aldehyde-Binding Protein 1
Otubain-1
Hotu1
Ubiquitin-Specific-Processing Protease Otub1
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-5689896
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ubiquitin Thioesterase Otub1 antibody
Anti-Deubiquitinating Enzyme Otub1 antibody
Anti-Otu Domain-Containing Ubiquitin Aldehyde-Binding Protein 1 antibody
Anti-Otubain-1 antibody
Anti-Hotu1 antibody
Anti-Ubiquitin-Specific-Processing Protease Otub1 antibody
Anti-OTUB1 antibody
Anti-OTB1 antibody
Anti-OTU1 antibody
Anti-HSPC263 antibody

Information sourced from Uniprot.org

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