| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Proteinase-activated receptor 2 (10-90 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
F2RL1 |
| Gene ID: |
2150 |
| Uniprot ID: |
PAR2_HUMAN |
| Immunogen Region: |
10-90 aa |
| Specificity: |
PAR2 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from the human protein at the amino acid range 10-90 |
| Post Translational Modifications | A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. Activating serine proteases include trypsin, mast cell tryptase, coagulation factors VII and Xa, myeloblastin/PRTN3 and membrane-type serine protease 1/ST14. Subsequent cleavage by serine proteases, including neutrophil elastase and cathepsin G, leads to receptor deactivation. At least in part, implicated proteases are also shown to activate the receptor.the glycosylation status of the receptor is thought to contribute to the difference. In addition to conventional trypsin-like proteases activated by other proteases and glycosidases derived from bacteria, fungi and insects. Activated by serine protease allergens such as dust mite Der p3 and Der p9 and mold Pen c13. Activated by P.gingivalis arginine-specific (trypsin-like) cysteine proteinases called gingipains. Activated by S.griseus exogenous chitinase. Activated by A.alternata aspartate protease.the cleavage generates non-conventional processed forms. N-glycosylated and sialylated. Multiple phosphorylated on serine and threonine residues in the cytoplasmic region upon receptor activation.required for receptor desensitization and recruitment of beta-arrestin. Monoubiquitinated by CBL at the plasma membrane and in early endosomes.not required for receptor endocytosis but for translocation to late endosomes or lysosomes. Deubiquitination involves STAMBP and USP8.required for lysosomal trafficking and receptor degradation. |
| Function | Receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Can also be transactivated by cleaved F2R/PAR1. Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection. Generally is promoting inflammation. Can signal synergistically with TLR4 and probably TLR2 in inflammatory responses and modulates TLR3 signaling. Has a protective role in establishing the endothelial barrier.the activity involves coagulation factor X. Regulates endothelial cell barrier integrity during neutrophil extravasation, probably following proteolytic cleavage by PRTN3. Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion. Involved in the regulation of vascular tone.activation results in hypotension presumably mediated by vasodilation. Associates with a subset of G proteins alpha subunits such as GNAQ, GNA11, GNA14, GNA12 and GNA13, but probably not with G(o)-alpha, G(i) subunit alpha-1 and G(i) subunit alpha-2. However, can signal through G(i) subunit alpha. Believed to be a class B receptor which internalizes as a complex with arrestin and traffic with it to endosomal vesicles, presumably as desensitized receptor, for extended periods of time. Mediates inhibition of TNF-alpha stimulated JNK phosphorylation via coupling to GNAQ and GNA11.the function involves dissociation of RIPK1 and TRADD from TNFR1. Mediates phosphorylation of nuclear factor NF-kappa-B RELA subunit at 'Ser-536'.the function involves IKBKB and is predominantly independent of G proteins. Involved in cellular migration. Involved in cytoskeletal rearrangement and chemotaxis through beta-arrestin-promoted scaffolds.the function is independent of GNAQ and GNA11 and involves promotion of cofilin dephosphorylation and actin filament severing. Induces redistribution of COPS5 from the plasma membrane to the cytosol and activation of the JNK cascade is mediated by COPS5. Involved in the recruitment of leukocytes to the sites of inflammation and is the major PAR receptor capable of modulating eosinophil function such as pro-inflammatory cytokine secretion, superoxide production and degranulation. During inflammation promotes dendritic cell maturation, trafficking to the lymph nodes and subsequent T-cell activation. Involved in antimicrobial response of innate immune cells.activation enhances phagocytosis of Gram-positive and killing of Gram-negative bacteria. Acts synergistically with interferon-gamma in enhancing antiviral responses. Implicated in a number of acute and chronic inflammatory diseases such as of the joints, lungs, brain, gastrointestinal tract, periodontium, skin, and vascular systems, and in autoimmune disorders. |
| Protein Name | Proteinase-Activated Receptor 2Par-2Coagulation Factor Ii Receptor-Like 1G-Protein Coupled Receptor 11Thrombin Receptor-Like 1 Cleaved Into - Proteinase-Activated Receptor 2 - Alternate Cleaved 1 - Proteinase-Activated Receptor 2 - Alternate Cleaved 2 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-416476 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Proteinase-Activated Receptor 2 antibodyAnti-Par-2 antibodyAnti-Coagulation Factor Ii Receptor-Like 1 antibodyAnti-G-Protein Coupled Receptor 11 antibodyAnti-Thrombin Receptor-Like 1 Cleaved Into - Proteinase-Activated Receptor 2 - Alternate Cleaved 1 - Proteinase-Activated Receptor 2 - Alternate Cleaved 2 antibodyAnti-F2RL1 antibodyAnti-GPR11 antibodyAnti-PAR2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
