• Immunofluorescence analysis of HepG2 cells, using PARD3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COLO205 cells, using PARD3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PARD3A Polyclonal Antibody

Anti-PARD3 antibody (1141-1190 aa) (STJ94955)

SKU:
STJ94955

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Partitioning defective 3 homolog (1141-1190 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PARD3
Gene ID: 56288
Uniprot ID: PARD3_HUMAN
Immunogen Region: 1141-1190 aa
Specificity: PARD3A Polyclonal Antibody detects endogenous levels of PARD3A protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PARD3 at the amino acid range 1141-1190
Post Translational Modifications Acetylated. Deacetylated by SIRT2, thereby inhibiting Schwann cell peripheral myelination. Phosphorylation at Ser-827 by PRKCZ and PRKCI occurs at the most apical tip of epithelial cell-cell contacts during the initial phase of tight junction formation and may promote dissociation of the complex with PARD6. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2. Phosphorylation by AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
Function Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
Protein Name Partitioning Defective 3 Homolog
Par-3
Pard-3
Atypical Pkc Isotype-Specific-Interacting Protein
Asip
Ctcl Tumor Antigen Se2-5
Par3-Alpha
Database Links Reactome: R-HSA-2173791
Reactome: R-HSA-420029
Cellular Localisation Cytoplasm
Endomembrane System
Cell Junction
Tight Junction
Adherens Junction
Cell Membrane
Cell Cortex
Cytoskeleton
Localized Along The Cell-Cell Contact Region
Colocalizes With Pard6a And Prkci At Epithelial Tight Junctions
Colocalizes With The Cortical Actin That Overlays The Meiotic Spindle During Metaphase I And Metaphase Ii
Colocalized With Sirt2 In Internode Region Of Myelin Sheath
Presence Of Krit1
Cdh5 And Rap1b Is Required For Its Localization To The Cell Junction
Alternative Antibody Names Anti-Partitioning Defective 3 Homolog antibody
Anti-Par-3 antibody
Anti-Pard-3 antibody
Anti-Atypical Pkc Isotype-Specific-Interacting Protein antibody
Anti-Asip antibody
Anti-Ctcl Tumor Antigen Se2-5 antibody
Anti-Par3-Alpha antibody
Anti-PARD3 antibody
Anti-PAR3 antibody
Anti-PAR3A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance