Anti-PARD3A antibody

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Product name Anti-PARD3A antibody
Description Rabbit polyclonal to PARD3A.
Applications ELISA, IF, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity PARD3A Polyclonal Antibody detects endogenous levels of PARD3A protein.
Protein Name Partitioning defective 3 homolog
PAR-3
PARD-3
Atypical PKC isotype-specific-interacting protein
ASIP
CTCL tumor antigen se2-5
PAR3-alpha
Immunogen Synthesized peptide derived from human PARD3A
Immunogen Region 1110-1190 aa, C-terminal
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 56288
Gene Symbol PARD3
Molecular Weight 151 kDa
Database Links HGNC:16051
OMIM:182940
Alternative Names Partitioning defective 3 homolog
PAR-3
PARD-3
Atypical PKC isotype-specific-interacting protein
ASIP
CTCL tumor antigen se2-5
PAR3-alpha
Function Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination . Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
Post-translational Modifications Acetylated. Deacetylated by SIRT2, thereby inhibiting Schwann cell peripheral myelination. Phosphorylation at Ser-827 by PRKCZ and PRKCI occurs at the most apical tip of epithelial cell-cell contacts during the initial phase of tight junction formation and may promote dissociation of the complex with PARD6. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2 . Phosphorylation by AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
Cellular Localization Cytoplasm Endomembrane system Cell junction Cell junction, tight junction Cell membrane Cytoplasm, cell cortex Cytoplasm, cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II. Colocalized with SIRT2 in internode region of myelin sheat . Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
Tissue Specificity Widely expressed.
Sequence and Domain Family Contains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization. The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
Swiss-Prot Key Q8TEW0_HUMAN
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