• Western blot analysis of extracts of various cell lines, using PARN antibody (STJ29021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 150s.
  • Immunofluorescence analysis of MCF7 cells using PARN antibody (STJ29021). Blue: DAPI for nuclear staining.

Anti-PARN antibody (1-280) (STJ29021)

SKU:
STJ29021

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PARN (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PARN
Gene ID: 5073
Uniprot ID: PARN_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PARN (NP_002573.1).
Immunogen Sequence: MEIIRSNFKSNLHKVYQAIE EADFFAIDGEFSGISDGPSV SALTNGFDTPEERYQKLKKH SMDFLLFQFGLCTFKYDYTD SKYITKSFNFYVFPKPFNRS SPDVKFVCQSSSIDFLASQG FDFNKVFRNGIPYLNQEEER QLREQYDEKRSQANGAGALS YVSPNTSKCPVTIPEDQKKF IDQVVEKIEDLLQSEENKNL DLEPCTGFQRKLIYQTLSWK YPKGIHVETLETEKKERYI
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylation by MAPKAPK2, preventing GADD45A mRNA degradation after genotoxic stress.
Function 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Also able to recognize and trim poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to microRNAs degradation or snoRNA increased stability.
Protein Name Poly(A-Specific Ribonuclease Parn
Deadenylating Nuclease
Deadenylation Nuclease
Polyadenylate-Specific Ribonuclease
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-429947
Reactome: R-HSA-450604
Cellular Localisation Nucleus
Cytoplasm
Nucleolus
Some Nuclear Fraction Is Nucleolar
Alternative Antibody Names Anti-Poly(A-Specific Ribonuclease Parn antibody
Anti-Deadenylating Nuclease antibody
Anti-Deadenylation Nuclease antibody
Anti-Polyadenylate-Specific Ribonuclease antibody
Anti-PARN antibody
Anti-DAN antibody

Information sourced from Uniprot.org

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