• Western blot analysis of lysates from 1) HepG2, 2) HuvEC, 3) COS7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Western blot analysis of lysates from HepG2 cells, using PCNA Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of COS7 cells using PCNA Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PCNA Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PCNA Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PCNA antibody (61-110 aa) (STJ94982)

SKU:
STJ94982

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey/Rabbit
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Proliferating cell nuclear antigen (61-110 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:100-500
IF ICC 1:100-500
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCNA
Gene ID: 5111
Uniprot ID: PCNA_HUMAN
Immunogen Region: 61-110 aa
Specificity: PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PCNA at the amino acid range 61-110
Function Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Protein Name Proliferating Cell Nuclear Antigen
Pcna
Cyclin
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-1362277
Reactome: R-HSA-174411
Reactome: R-HSA-174414
Reactome: R-HSA-174417
Reactome: R-HSA-174437
Reactome: R-HSA-4615885
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685942
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6804114
Reactome: R-HSA-69091
Reactome: R-HSA-69166
Reactome: R-HSA-69183
Reactome: R-HSA-69205
Reactome: R-HSA-8866654
Cellular Localisation Nucleus
Colocalizes With Crebbp
Ep300 And Pold1 To Sites Of Dna Damage
Forms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S Phase
Co-Localizes With Smarca5/Snf2h And Baz1b/Wstf At Replication Foci During S Phase
Together With Apex2
Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents
Alternative Antibody Names Anti-Proliferating Cell Nuclear Antigen antibody
Anti-Pcna antibody
Anti-Cyclin antibody
Anti-PCNA antibody

Information sourced from Uniprot.org

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