• Western blot analysis of extracts of various cell lines, using PDE5A antibody (STJ28913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PDE5A antibody (1-150) (STJ28913)

SKU:
STJ28913

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PDE5A (1-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PDE5A
Gene ID: 8654
Uniprot ID: PDE5A_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human PDE5A (NP_001074.2).
Immunogen Sequence: MERAGPSFGQQRQQQQPQQQ KQQQRDQDSVEAWLDDHWDF TFSYFVRKATREMVNAWFAE RVHTIPVCKEGIRGHTESCS CPLQQSPRADNSAPGTPTRK ISASEFDRPLRPIVVKDSEG TVSFLSDSEKKEQMPLTPPR FDHDEGDQCS
Tissue Specificity Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
Post Translational Modifications Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Phosphorylation by PRKG1 leads to its activation.
Function Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.
Protein Name Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase
Cgmp-Binding Cgmp-Specific Phosphodiesterase
Cgb-Pde
Database Links Reactome: R-HSA-418457
Reactome: R-HSA-445355
Reactome: R-HSA-9013422
Alternative Antibody Names Anti-Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase antibody
Anti-Cgmp-Binding Cgmp-Specific Phosphodiesterase antibody
Anti-Cgb-Pde antibody
Anti-PDE5A antibody
Anti-PDE5 antibody

Information sourced from Uniprot.org

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