• Western blot analysis of extracts of various cell lines, using PEX2 antibody (STJ117503) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Immunohistochemistry of paraffin-embedded human placenta using PEX2 rabbit polyclonal antibody (STJ117503) at dilution of 1:100 (40x lens).

Anti-PEX2 antibody (1-130) (STJ117503)

SKU:
STJ117503

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PEX2 (1-130) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PEX2
Gene ID: 5828
Uniprot ID: PEX2_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PEX2 (NP_001165558.1).
Immunogen Sequence: MASRKENAKSANRVLRISQL DALELNKALEQLVWSQFTQC FHGFKPGLLARFEPEVKACL WVFLWRFTIYSKNATVGQSV LNIKYKNDFSPNLRYQPPSK NQKIWYAVCTIGGRWLEERC YDLFRNHHLA
Post Translational Modifications Forms intramolecular and intermolecular disulfide bonds in response to reactive oxygen species (ROS), promoting higher stability.
Function E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12.each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase. PEX2 ubiquitinates PEX5 during its passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 at 'Cys-11', a modification that acts as a signal for PEX5 extraction into the cytosol. Required for pexophagy in response to starvation by mediating ubiquitination of peroxisomal proteins, such as PEX5 and ABCD3/PMP70. Also involved in the response to reactive oxygen species (ROS) by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of PNPLA2/ATGL, thereby regulating lipolysis.
Protein Name Peroxisome Biogenesis Factor 2
35 Kda Peroxisomal Membrane Protein
Peroxin-2
Peroxisomal Membrane Protein 3
Peroxisome Assembly Factor 1
Paf-1
Ring Finger Protein 72
Database Links Reactome: R-HSA-8866654
Reactome: R-HSA-9033241
Reactome: R-HSA-9603798
Cellular Localisation Peroxisome Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Peroxisome Biogenesis Factor 2 antibody
Anti-35 Kda Peroxisomal Membrane Protein antibody
Anti-Peroxin-2 antibody
Anti-Peroxisomal Membrane Protein 3 antibody
Anti-Peroxisome Assembly Factor 1 antibody
Anti-Paf-1 antibody
Anti-Ring Finger Protein 72 antibody
Anti-PEX2 antibody
Anti-PAF1 antibody
Anti-PMP3 antibody
Anti-PMP35 antibody
Anti-PXMP3 antibody
Anti-RNF72 antibody

Information sourced from Uniprot.org

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