Anti-Phospho-AKT (Thr342) antibody (KLH)

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Product name Anti-Phospho-AKT (Thr342) antibody (KLH)
Short Description Rabbit polyclonal against Phospho-AKT (Thr342) (KLH)
Description The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (alpha, beta, and gamma) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktalpha occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004).
Applications WB
Dilution range WB 1:500
Protein Name RAC serine/threonine-protein kinase
Protein kinase B
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 conjugated to KLH.
Storage Instruction Store at -20°C, and avoid repeated freeze-thaw cycles.
Note For research use only (RUO).
Validated Application WB
Host Rabbit
Clonality Polyclonal
Conjugation KLH
Purification Anti-Phospho-AKT (Thr342) antibody (KLH) was prepared using affinity purification from Pooled Serum
Formulation 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Molecular Weight 68 kDa
Alternative Names RAC serine/threonine-protein kinase
Protein kinase B
Function Serine/threonine kinase involved in various developmental processes. During early embryogenesis, acts as a survival protein. During mid-embryogenesis, phosphorylates and activates trh, a transcription factor required for tracheal cell fate determination. Also regulates tracheal cell migration. Later in development, acts downstream of PI3K and Pk61C/PDK1 in the insulin receptor transduction pathway which regulates cell growth and organ size, by phosphorylating and antagonizing FOXO transcription factor. Controls follicle cell size during oogenesis. May also stimulate cell growth by phosphorylating Gig/Tsc2 and inactivating the Tsc complex. Dephosphorylation of 'Ser-586' by Phlpp triggers apoptosis and suppression of tumor growth.
Post-translational Modifications Phosphorylated and activated by Pk61C/PDK1. Phosphorylated on Ser-586 by the rictor-Tor complex.
Cellular Localization Cytoplasm, cytosol. Cell membrane. Note: Recruited to plasma membrane upon activation.
Tissue Specificity Ubiquitously expressed. Present in ovary, where it was concentrated at the basal side of follicle cells.
Sequence and Domain Family Belongs to the 14-3-10 family.Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
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