• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ERK1-Y204 antibody (STJ22262) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g Phospho-ERK1-Y204 polyclonal antibody (STJ22262). Western blot was performed from the immunoprecipitate using Phospho-ERK1-Y204 polyclonal antibody (STJ22262) at a dilution of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes.
  • Immunohistochemistry of paraffin-embedded rat liver using Phospho-ERK1-Y204 antibody (STJ22262) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of methanol-fixed HeLa cells showing centrosome and nuclear staining using Phospho-ERK1-Y204 Rabbit polyclonal antibody (STJ22262).
  • Immunohistochemistry of paraffin-embedded human uterus using Phospho-ERK1-Y204 antibody (STJ22262) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-ERK1-Y204 in paraffin-embedded mouse heart using Phospho-ERK1-Y204 Rabbit polyclonal antibody (STJ22262) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse heart using Phospho-ERK1-Y204 antibody (STJ22262) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-ERK1-Y204 in paraffin-embedded human uterus using Phospho-ERK1-Y204 Rabbit polyclonal antibody (STJ22262) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of methanol-fixed HeLa cells showing centrosome and nuclear staining using Phospho-ERK1-Y204 antibody (STJ22262).
  • Immunohistochemistry analysis of Phospho-ERK1-Y204 in paraffin-embedded rat liver using Phospho-ERK1-Y204 Rabbit polyclonal antibody (STJ22262) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ERK1-Y204 polyclonal antibody (STJ22262). Western blot was performed from the immunoprecipitate using Phospho-ERK1-Y204 polyclonal antibody (STJ22262) at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ERK1-Y204 Rabbit polyclonal antibody (STJ22262) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-MAPK3-Y204 antibody (STJ22262)

SKU:
STJ22262

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MAPK3-Y204 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:100-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK3
Gene ID: 5595
Uniprot ID: MK03_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y204 of human ERK1 (NP_002737.2).
Immunogen Sequence: TEYVA
Post Translational Modifications Phosphorylated upon KIT and FLT3 signaling. Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204. Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination.leading to activation. Deubiquitinated by CYLD.
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Protein Name Mitogen-Activated Protein Kinase 3
Map Kinase 3
Mapk 3
Ert2
Extracellular Signal-Regulated Kinase 1
Erk-1
Insulin-Stimulated Map2 Kinase
Map Kinase Isoform P44
P44-Mapk
Microtubule-Associated Protein 2 Kinase
P44-Erk1
Database Links Reactome: R-HSA-110056
Reactome: R-HSA-112409
Reactome: R-HSA-1169408
Reactome: R-HSA-1181150
Reactome: R-HSA-1295596
Reactome: R-HSA-1502540
Reactome: R-HSA-162658 P27361-3
Reactome: R-HSA-170968
Reactome: R-HSA-198753
Reactome: R-HSA-202670
Reactome: R-HSA-2029482
Reactome: R-HSA-2173795
Reactome: R-HSA-2173796
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-2871796
Reactome: R-HSA-3371453
Reactome: R-HSA-375165
Reactome: R-HSA-444257
Reactome: R-HSA-445144
Reactome: R-HSA-450341
Reactome: R-HSA-456926
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5663213
Reactome: R-HSA-5668599
Reactome: R-HSA-5673001
Reactome: R-HSA-5674135
Reactome: R-HSA-5674499
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-6811558
Reactome: R-HSA-73728
Reactome: R-HSA-74749
Reactome: R-HSA-877300
Reactome: R-HSA-879415
Reactome: R-HSA-881907
Reactome: R-HSA-8939211
Reactome: R-HSA-8940973
Reactome: R-HSA-8943724
Reactome: R-HSA-9627069
Reactome: R-HSA-9634638
Reactome: R-HSA-9635465
Reactome: R-HSA-9649948
Reactome: R-HSA-9652169
Reactome: R-HSA-9656223
Reactome: R-HSA-9664422
Reactome: R-HSA-9725371
Reactome: R-HSA-9732724
Reactome: R-HSA-9768919
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Nucleus
Membrane
Caveola
Cell Junction
Focal Adhesion
Autophosphorylation At Thr-207 Promotes Nuclear Localization
Pea15-Binding Redirects The Biological Outcome Of Mapk3 Kinase-Signaling By Sequestering Mapk3 Into The Cytoplasm
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 3 antibody
Anti-Map Kinase 3 antibody
Anti-Mapk 3 antibody
Anti-Ert2 antibody
Anti-Extracellular Signal-Regulated Kinase 1 antibody
Anti-Erk-1 antibody
Anti-Insulin-Stimulated Map2 Kinase antibody
Anti-Map Kinase Isoform P44 antibody
Anti-P44-Mapk antibody
Anti-Microtubule-Associated Protein 2 Kinase antibody
Anti-P44-Erk1 antibody
Anti-MAPK3 antibody
Anti-ERK1 antibody
Anti-PRKM3 antibody

Information sourced from Uniprot.org

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