• Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K7 (Phospho-Ser271) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from NIH/3T3 cells treated with insulin 0.01U/ml 15' and NIH/3T3 cells treated with EGF 200ng/ml 30', using MAP2K7 (Phospho-Ser271) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 3T3 cells using Phospho-MEK-7 (S271) Polyclonal Antibody

Anti-Phospho-MAP2K7-Ser271 antibody (236-285 aa) (STJ90950)

SKU:
STJ90950

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Dual specificity mitogen-activated protein kinase kinase 7-Ser271 (236-285 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K7
Gene ID: 5609
Uniprot ID: MP2K7_HUMAN
Immunogen Region: 236-285 aa
Specificity: Phospho-MEK-7 (S271) Polyclonal Antibody detects endogenous levels of MEK-7 protein only when phosphorylated at S271.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MAP2K7 around the phosphorylation site of Ser271 at the amino acid range 236-285
Post Translational Modifications Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 7
Map Kinase Kinase 7
Mapkk 7
Jnk-Activating Kinase 2
Mapk/Erk Kinase 7
Mek 7
Stress-Activated Protein Kinase Kinase 4
Sapk Kinase 4
Sapkk-4
Sapkk4
C-Jun N-Terminal Kinase Kinase 2
Jnk Kinase 2
Jnkk 2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2871796
Reactome: R-HSA-450321
Reactome: R-HSA-5210891
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 7 antibody
Anti-Map Kinase Kinase 7 antibody
Anti-Mapkk 7 antibody
Anti-Jnk-Activating Kinase 2 antibody
Anti-Mapk/Erk Kinase 7 antibody
Anti-Mek 7 antibody
Anti-Stress-Activated Protein Kinase Kinase 4 antibody
Anti-Sapk Kinase 4 antibody
Anti-Sapkk-4 antibody
Anti-Sapkk4 antibody
Anti-C-Jun N-Terminal Kinase Kinase 2 antibody
Anti-Jnk Kinase 2 antibody
Anti-Jnkk 2 antibody
Anti-MAP2K7 antibody
Anti-JNKK2 antibody
Anti-MEK7 antibody
Anti-MKK7 antibody
Anti-PRKMK7 antibody
Anti-SKK4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance