• Western blot analysis of MCF-7 cells using Phospho-RPA p32 (S33) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of lysates from NIH/3T3 cells treated with Adriamycin 0.5ug/ml 24h, using RFA2 (Phospho-Ser33) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-RPA p32 (S33) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-RPA2-Ser33 antibody (1-50 aa) (STJ90636)

SKU:
STJ90636

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Replication protein A 32 kDa subunit-Ser33 (1-50 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPA2
Gene ID: 6118
Uniprot ID: RFA2_HUMAN
Immunogen Region: 1-50 aa
Specificity: Phospho-RPA p32 (S33) Polyclonal Antibody detects endogenous levels of RPA p32 protein only when phosphorylated at S33.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RFA2 around the phosphorylation site of Ser33 at the amino acid range 1-50
Function As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Protein Name Replication Protein A 32 Kda Subunit
Rp-A P32
Replication Factor A Protein 2
Rf-A Protein 2
Replication Protein A 34 Kda Subunit
Rp-A P34
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-174437
Reactome: R-HSA-176187
Reactome: R-HSA-3371453
Reactome: R-HSA-3371511
Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-5696395
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6783310
Reactome: R-HSA-6804756
Reactome: R-HSA-68962
Reactome: R-HSA-69166
Reactome: R-HSA-69473
Reactome: R-HSA-912446
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Pml Body
Redistributes To Discrete Nuclear Foci Upon Dna Damage In An Atr-Dependent Manner
Alternative Antibody Names Anti-Replication Protein A 32 Kda Subunit antibody
Anti-Rp-A P32 antibody
Anti-Replication Factor A Protein 2 antibody
Anti-Rf-A Protein 2 antibody
Anti-Replication Protein A 34 Kda Subunit antibody
Anti-Rp-A P34 antibody
Anti-RPA2 antibody
Anti-REPA2 antibody
Anti-RPA32 antibody
Anti-RPA34 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance