Anti-PI 3-kinase p8 alpha/gamma antibody

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Product name Anti-PI 3-kinase p8 alpha/gamma antibody
Short Description Rabbit polyclonal against PI 3-kinase p8 alpha/gamma
Description PI 3-kinase p85alpha/gamma is a protein encoded by the PIK3R1 gene which is approximately 83,5 kDa. PI 3-kinase p85alpha/gamma is localised to the cytoplasm, nucleus and cis-Golgi network. It is involved in RET signalling, the IL-2 pathway, downstream signalling of activated FGFR2 and regulation of lipid metabolism. It binds to activated protein-Tyr kinases, through its SH2 domain, and acts as an adapter by mediating the association of the p110 catalytic unit to the plasma membrane. It is also necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. PI 3-kinase p85alpha/gamma isoform 2 is expressed in skeletal muscle and brain. Mutations in the PIK3R1 gene may result in Short syndrome and Pasli disease. STJ95078 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PI 3-kinase p85alpha/gamma protein.
Applications ELISA, IF, IHC, WB
Dilution range WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity PI 3-kinase p85alpha/gamma Polyclonal Antibody detects endogenous levels of PI 3-kinase p85alpha/gamma protein.
Protein Name Phosphatidylinositol 3-kinase regulatory subunit alpha
PI3-kinase regulatory subunit alpha
PI3K regulatory subunit alpha
PtdIns-3-kinase regulatory subunit alpha
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
P
Immunogen Synthesized peptide derived from human PI 3-kinase p85/p55 around the non-phosphorylation site of Y458/199.
Immunogen Region 410-490 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IF IHC WB
Host Rabbit
Clonality Polyclonal
Reactivity Avian, Human, Mouse, Rat, Simian
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 5295
Gene Symbol PIK3R1
Molecular Weight 83/54 kDa
Database Links HGNC:8979
OMIM:171833
Alternative Names Phosphatidylinositol 3-kinase regulatory subunit alpha
PI3-kinase regulatory subunit alpha
PI3K regulatory subunit alpha
PtdIns-3-kinase regulatory subunit alpha
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
P
Function Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling . Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement .
Post-translational Modifications Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear . Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.
Tissue Specificity Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
Sequence and Domain Family The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
Swiss-Prot Key P27986_HUMAN
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