• Western blot analysis of extracts of various cell lines, using PLD2 antibody (STJ112230) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PLD2 antibody (1-180) (STJ112230)

SKU:
STJ112230

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLD2 (1-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLD2
Gene ID: 5338
Uniprot ID: PLD2_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PLD2 (NP_002654.3).
Immunogen Sequence: MTATPESLFPTGDELDSSQL QMESDEVDTLKEGEDPADRM HPFLAIYELQSLKVHPLVFA PGVPVTAQVVGTERYTSGSK VGTCTLYSVRLTHGDFSWTT KKKYRHFQELHRDLLRHKVL MSLLPLARFAVAYSPARDAG NREMPSLPRAGPEGSTRHAA SKQKYLENYLNRLLTMSFYR
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated by FGR.
Function Function as phospholipase selective for phosphatidylcholine. May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Protein Name Phospholipase D2
Pld 2
Hpld2
Choline Phosphatase 2
Pld1c
Phosphatidylcholine-Hydrolyzing Phospholipase D2
Database Links Reactome: R-HSA-1483148
Reactome: R-HSA-1483166
Reactome: R-HSA-2029485
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Cellular Localisation Cell Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Phospholipase D2 antibody
Anti-Pld 2 antibody
Anti-Hpld2 antibody
Anti-Choline Phosphatase 2 antibody
Anti-Pld1c antibody
Anti-Phosphatidylcholine-Hydrolyzing Phospholipase D2 antibody
Anti-PLD2 antibody

Information sourced from Uniprot.org

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