• Immunohistochemistry analysis of PLEKHB2 in paraffin-embedded Human liver cancer using PLEKHB2 Rabbit polyclonal antibody (STJ117657) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PLEKHB2 Rabbit polyclonal antibody (STJ117657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PLEKHB2 antibody (1-222) (STJ117657)

SKU:
STJ117657

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLEKHB2 (1-222) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLEKHB2
Gene ID: 55041
Uniprot ID: PKHB2_HUMAN
Immunogen Region: 1-222
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human PLEKHB2 (NP_001094093.1).
Immunogen Sequence: MAFVKSGWLLRQSTILKRWK KNWFDLWSDGHLIYYDDQTR QNIEDKVHMPMDCINIRTGQ ECRDTQPPDGKSKDCMLQIV CRDGKTISLCAESTDDCLAW KFTLQDSRTNTAYVGSAVMT DETSVVSSPPPYTAYAAPAP EQAYGYGPYGGAYPPGTQVV YAANGQAYAVPYQYPYAGLY GQQPANQVIIRERYRDNDSD LALGMLAGAATGMALGSLFW VF
Function Involved in retrograde transport of recycling endosomes.
Protein Name Pleckstrin Homology Domain-Containing Family B Member 2
Ph Domain-Containing Family B Member 2
Evectin-2
Cellular Localisation Recycling Endosome Membrane
Peripheral Membrane Protein
Specifically Detected In Tubulovesicular Structures
And Colocalizes With Tfnr
Alternative Antibody Names Anti-Pleckstrin Homology Domain-Containing Family B Member 2 antibody
Anti-Ph Domain-Containing Family B Member 2 antibody
Anti-Evectin-2 antibody
Anti-PLEKHB2 antibody
Anti-EVT2 antibody

Information sourced from Uniprot.org

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