• Western blot analysis of extracts of various cell lines, using PLIN3 antibody (STJ116409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PLIN3 antibody (135-434) (STJ116409)

SKU:
STJ116409

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLIN3 (135-434) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLIN3
Gene ID: 10226
Uniprot ID: PLIN3_HUMAN
Immunogen Region: 135-434
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 135-434 of human PLIN3 (NP_005808.3).
Immunogen Sequence: MVSSAKDTVATQLSEAVDAT RGAVQSGVDKTKSVVTGGVQ SVMGSRLGQMVLSGVDTVLG KSEEWADNHLPLTDAELARI ATSLDGFDVASVQQQRQEQS YFVRLGSLSERLRQHAYEHS LGKLRATKQRAQEALLQLSQ VLSLMETVKQGVDQKLVEGQ EKLHQMWLSWNQKQLQGPEK EPPKPEQVESRALTMFRDIA QQLQATCTSLGSSIQGLPTN VKDQVQQARRQVEDLQATF
Post Translational Modifications Phosphorylation at Tyr-251 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis.
Function Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets. Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
Protein Name Perilipin-3
47 Kda Mannose 6-Phosphate Receptor-Binding Protein
47 Kda Mpr-Binding Protein
Cargo Selection Protein Tip47
Mannose-6-Phosphate Receptor-Binding Protein 1
Placental Protein 17
Pp17
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-6811440
Reactome: R-HSA-9613354
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9706019
Cellular Localisation Lipid Droplet
Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Membrane Associated On Endosomes
Detected In The Envelope And The Core Of Lipid Bodies And In Lipid Sails
Alternative Antibody Names Anti-Perilipin-3 antibody
Anti-47 Kda Mannose 6-Phosphate Receptor-Binding Protein antibody
Anti-47 Kda Mpr-Binding Protein antibody
Anti-Cargo Selection Protein Tip47 antibody
Anti-Mannose-6-Phosphate Receptor-Binding Protein 1 antibody
Anti-Placental Protein 17 antibody
Anti-Pp17 antibody
Anti-PLIN3 antibody
Anti-M6PRBP1 antibody
Anti-TIP47 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance